DNA analysis for section identification of individual Pinus pollen grains from Belukha glacier, Altai Mountains, Russia
DNA analysis for section identification of individual Pinus pollen grains from Belukha glacier, Altai Mountains, Russia
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对来自俄罗斯阿尔泰山脉别卢哈冰川的单个松属花粉粒进行切片鉴定的 DNA 分析
DOI：
10.1088/1748-9326/8/1/014032
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Hiroshi Kanda
中科院分区：
文献类型：
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作者：
Fumio Nakazawa;Jun Uetake;Yoshihisa Suyama;Ryo Kaneko;Nozomu Takeuchi;Koji Fujita;Hideaki Motoyama;Satoshi Imura;Hiroshi Kanda
沉积物样品中的花粉分类单元可以通过分析花粉形态来识别。根据花粉形态鉴定相关物种很困难，并且主要限于属或科。由于不同年龄的花粉粒在冰川中保存在 0°C 以下，因此更有可能保持完整或几乎没有 DNA 断裂，因此来自此类花粉粒的遗传信息应该能够识别属水平以下的植物分类单元。然而，尚未发表的研究尝试使用从冰川中发现的花粉中获得的 DNA 序列进行详细鉴定。作为初步步骤，本研究尝试分析 2003 年夏季从俄罗斯阿尔泰山别卢哈冰川收集的表层雪中提取的松树花粉粒的 DNA。通过聚合酶链式反应扩增每个松树花粉粒叶绿体基因组的 150 bp rpoB 片段，并对 DNA 产物进行测序以在切片水平上进行鉴定。总共 105 个花粉粒用于测试，并从 8 个花粉粒中获得了序列。根据获得的序列，花粉粒被鉴定为属于五叶草科。目前在冰川周围发现了五叶松科现存西伯利亚松树种。本部分结果的一致性表明，冰川中的花粉源自与周围环境中发现的相同的松树。
Pollen taxon in sediment samples can be identified by analyzing pollen morphology. Identification of related species based on pollen morphology is difficult and is limited primarily to genus or family. Because pollen grains of various ages are preserved at below 0 C in glaciers and thus are more likely to remain intact or to suffer little DNA fragmentation, genetic information from such pollen grains should enable identification of plant taxa below the genus level. However, no published studies have attempted detailed identification using DNA sequences obtained from pollen found in glaciers. As a preliminary step, this study attempted to analyze the DNA of Pinus pollen grains extracted from surface snow collected from the Belukha glacier in the Altai Mountains of Russia in the summer of 2003. A 150-bp rpoB fragment from the chloroplast genome in each Pinus pollen grain was amplified by polymerase chain reaction, and DNA products were sequenced to identify them at the section level. A total of 105 pollen grains were used for the test, and sequences were obtained from eight grains. From the sequences obtained, the pollen grains were identified as belonging to the section Quinquefoliae. Trees of the extant species Pinus sibirica in the section Quinquefoliae are currently found surrounding the glacier. The consistency of results for this section suggests that the pollen in the glacier originated from the same Pinus trees as those found in the immediate surroundings.