Identification and production of a novel natural pigment, cordycepoid A, from Cordyceps bifusispora
Identification and production of a novel natural pigment, cordycepoid A, from Cordyceps bifusispora
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双孢冬虫夏草天然色素虫草素 A 的鉴定及制备
DOI：
10.1007/s00253-013-4966-y
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发表时间：
2013-07-01
影响因子：
5
通讯作者：
Bao, Guan-Hu
中科院分区：
文献类型：
--
作者：
Lu, Rui-Li;Luo, Fei-Fei;Bao, Guan-Hu
从虫生真菌双梭孢虫草（Cordyceps bifusispora）中分离并鉴定了一种新的黄色素--虫草素A（Cordycepoid A）。虫草素A对中国仓鼠卵巢（CHO）细胞和小鼠无明显毒性，对食品添加剂、金属离子和热稳定性高。用于生产该色素的液/固两相培养工艺优化如下：3天老化的液体种子，7.5%的接种物，25 ℃的孵育温度，10天的固体培养，最后5天暴露于200 Lx散射光。对液体种子培养基和固体培养基进行了优化。选择乙醇作为提取溶剂进行放大生产。结果表明，最佳提取工艺条件为：料液比20：1，提取温度40 A ℃，超声功率400 W，提取时间40 min。
A novel yellow pigment, cordycepoid A, was isolated and identified from the entomogenous fungi Cordyceps bifusispora. Cordycepoid A exhibited no significant toxicity against Chinese hamster ovary (CHO) cells and mice, and showed high stability against food addictives, metal ions and heat. A liquid/solid double-phase cultural process for the production of the pigment was optimized as follows: 3 days aged liquid seed, 7.5 % inoculums, incubation temperature at 25 A degrees C, 10 days of solid culture, and the last 5 days exposed to 200 Lx scattered light. The liquid seed medium and the solid culture medium were also optimized. Ethanol was selected as extracting solvent for its scale-up production. The optimal extracting conditions were determined as liquid/solid ratio at 20:1, extracting temperature at 40 A degrees C, ultrasonic power at 400 W, and extracting time of 40 min.