Insight into the biology of Mycobacterium mucogenicum and Mycobacterium neoaurum Glade members
Insight into the biology of Mycobacterium mucogenicum and Mycobacterium neoaurum Glade members
复制标题
DOI：
10.1038/s41598-019-55464-5
复制
发表时间：
2019-12-17
影响因子：
4.6
通讯作者：
Kirsebom, Leif A.
中科院分区：
文献类型：
--
作者：
Behra, Phani Rama Krishna;Pettersson, B. M. Fredrik;Kirsebom, Leif A.
非结核分枝杆菌（NTM）越来越受到关注，并且在这些成员中，粘液生成分枝杆菌（Mmuc）和新金分枝杆菌（Mneo）分支可引起人类感染，并且它们对一线抗结核药物具有抗性。它们可以从土壤、自来水和地下水等不同的生态位中分离出来。分枝杆菌，如Mmuc和Mneo，被归类为快速生长的分枝杆菌，RGM，而最熟悉的结核分枝杆菌，属于缓慢生长的分枝杆菌，SGM。现代“组学”方法为我们理解这组细菌的生物学和进化提供了新的见解。在这里，我们提出了比较基因组学数据的17个NTM，其中16个属于Mmuc和Mneo分支。专注于毒力基因，包括编码西格玛/反西格玛因子，丝氨酸苏氨酸蛋白激酶（STPK），VII型（ESX基因）分泌系统和哺乳动物细胞进入（Mce）因子的基因，我们提供洞察他们的存在以及系统发育关系的情况下，西格玛/反西格玛因子和STPK。我们的数据进一步表明，这些NTM缺乏ESX-5和Mce2基因，这是已知的影响毒力。在这种情况下，Mmuc-和Mneo-进化枝成员缺乏糖肽脂（GLP）基因座中的几个基因，这些基因在菌落形态型外观和毒力中起作用。对于M.在Mmucogenicum型菌株Mmuc（T）中，我们提供了专注于σ因子、STPK、ESX蛋白和Mce蛋白的mRNA水平的RNASeq数据。这些数据进行了讨论和比较，特别是SGM和鱼类病原体海洋分枝杆菌。最后，我们提供了深入了解为什么Mmuc和Mneo进化枝的成员对利福平和异烟肼表现出耐药性，以及为什么Mmuc（T）形成粗糙的菌落形态型。
Nontuberculous mycobacteria, NTM, are of growing concern and among these members of the Mycobacterium mucogenicum (Mmuc) and Mycobacterium neoaurum (Mneo) clades can cause infections in humans and they are resistant to first-line anti-tuberculosis drugs. They can be isolated from different ecological niches such as soil, tap water and ground water. Mycobacteria, such as Mmuc and Mneo, are classified as rapid growing mycobacteria, RGM, while the most familiar, Mycobacterium tuberculosis, belongs to the slow growing mycobacteria, SGM. Modern "omics" approaches have provided new insights into our understanding of the biology and evolution of this group of bacteria. Here we present comparative genomics data for seventeen NTM of which sixteen belong to the Mmuc- and Mneo-clades. Focusing on virulence genes, including genes encoding sigma/anti-sigma factors, serine threonine protein kinases (STPK), type VII (ESX genes) secretion systems and mammalian cell entry (Mce) factors we provide insight into their presence as well as phylogenetic relationship in the case of the sigma/anti-sigma factors and STPKs. Our data further suggest that these NTM lack ESX-5 and Mce2 genes, which are known to affect virulence. In this context, Mmuc- and Mneo-clade members lack several of the genes in the glycopeptidolipid (GLP) locus, which have roles in colony morphotype appearance and virulence. For the M. mucogenicum type strain, Mmuc(T), we provide RNASeq data focusing on mRNA levels for sigma factors, STPK, ESX proteins and Mce proteins. These data are discussed and compared to in particular the SGM and fish pathogen Mycobacterium marinum. Finally, we provide insight into as to why members of the Mmuc- and Mneo-clades show resistance to rifampin and isoniazid, and why Mmuc(T) forms a rough colony morphotype.