Marek's disease virus down-regulates surface expression of MHC (B complex) class 1 (BF) glycoproteins during active but not latent infection of chicken cells
Marek's disease virus down-regulates surface expression of MHC (B complex) class 1 (BF) glycoproteins during active but not latent infection of chicken cells
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DOI：
10.1006/viro.2000.0797
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发表时间：
2001-03-30
期刊：
影响因子：
3.7
通讯作者：
Parcells, MS
中科院分区：
文献类型：
--
作者：
Hunt, HD;Lupiani, B;Parcells, MS
马立克氏病病毒(MDV)三种血清型感染鸡细胞后，其主要组织相容性复合体(MHC或B复合体)I类(BF)糖蛋白的表达受到干扰。MDV血清型1、2和3型感染OU2细胞后，BF表面表达被阻断。MDV诱导的T细胞肿瘤在5-溴-2‘-脱氧尿苷(BUDR)重新激活病毒后，细胞表面BF几乎完全丧失。转化MDCC-UA04细胞的重组病毒(RB1BUS2gfp Delta)在病毒基因表达的早期表达绿色荧光蛋白(GFP)。在被诱导表达早期GFP的UA04细胞中，大约60%的细胞BF表达水平降低。所有重新激活的表达主要早期病毒蛋白pp38的UA04和MSB1肿瘤细胞都显示出BF水平降低。因此，BF的下调开始于早期阶段，并在病毒基因表达的早期阶段完成。细胞内的BF池没有受到明显的影响，这表明可能的机制是BF转运受阻，而不是转录或翻译调节的结果。(C)2001年学术出版社。
Infection of chicken cells with three Marek's disease Virus (MDV) serotypes interferes with expression of the major histocompatibility complex (MHC or B complex) class I (BF) glycoproteins. BF surface expression is blocked after infection of OU2 cells with MDV serotypes 1, 2, and 3. MDV-induced T-cell tumors suffer a nearly complete loss of cell surface BF upon virus reactivation with 5-bromo-2'-deoxyuridine (BUdR). The recombinant virus (RB1BUS2gfp Delta) transforming the MDCC-UA04 cell line expresses green fluorescent protein (GFP) during the immediate early phase of viral gene expression. Of the UA04 cells induced to express the immediate early GFP, approximately 60% have reduced levels of BF expression. All of the reactivated UA04 and MSB1 tumor cells expressing the major early viral protein pp38 display reduced revels of BF. Thus, BF down-regulation begins in the immediate early phase and is complete by the early phase of viral gene expression. The intracellular pool of BF is not appreciably affected, indicating that the likely mechanism is a block in BF transport and not the result of transcriptional or translational regulation. (C) 2001 Academic Press.