Neuroglycan C, a neural tissue-specific transmembrane chondroitin sulfate proteoglycan, in retinal neural network formation.
Neuroglycan C, a neural tissue-specific transmembrane chondroitin sulfate proteoglycan, in retinal neural network formation.
复制标题
Neuroglycan C，一种神经组织特异性跨膜硫酸软骨素蛋白聚糖，参与视网膜神经网络的形成。
DOI：
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发表时间：
2000
影响因子：
4.4
通讯作者：
Y. Honda
中科院分区：
文献类型：
--
作者：
M. Inatani;H. Tanihara;A. Oohira;Y. Otori;A. Nishida;M. Honjo;N. Kido;Y. Honda
目的
神经聚糖C（NGC）是一种跨膜硫酸软骨素蛋白聚糖，仅存在于中枢神经系统组织中。在本研究中，NGC在视网膜发育过程中的表达模式和特征进行了研究。
方法
采用半定量逆转录-聚合酶链反应（RT-PCR）检测NGC mRNA在大鼠视网膜发育过程中的表达变化。使用抗NGC抗体通过免疫印迹分析和免疫组织化学研究NGC核心蛋白的定位和表征。
结果
免疫组化结果显示，NGC在大鼠出生后视网膜发育过程中的神经纤维层（NFL）和内丛状层（IPL）有高表达。在胚胎期，NGC免疫反应微弱。相反，在出生后发育阶段（大约出生后第7天[P]），在NFL和IPL中观察到强烈的免疫反应性，在那里观察到活跃的树突分支，并开始形成常规突触。随着视网膜层分化的进行（从P14到P42），视网膜内层的免疫反应逐渐减弱。免疫印迹和半定量RT-PCR分析表明，NGC表达的峰值水平发生在约P7和P14。随着视网膜层的成熟，NGC核心蛋白的糖基化发生变化。在免疫电镜分析中，NGC免疫反应性位于轴突膜的神经元细胞在出生后的视网膜，而免疫反应性降低膜在成人阶段。在体外培养的视网膜神经节细胞中，NGC高度定位于其多刺出芽的神经突中。
结论
结果显示了NGC的时空表达模式，并表明它在视网膜发育中的神经网络的形成中起作用。
PURPOSE
Neuroglycan C (NGC) is a transmembrane chondroitin sulfate proteoglycan present exclusively in central nervous system tissues. In the current study the expression pattern and characterization of NGC during the development of the retina were investigated.
METHODS
Expressional changes of NGC mRNAs during rat retinal development were examined by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The localization and characterization of NGC core proteins were investigated by immunoblot analysis and immunohistochemistry using an anti-NGC antibody.
RESULTS
Immunohistochemical analysis revealed that NGC was highly expressed in the nerve fiber layer (NFL) and inner plexiform layer (IPL) in rat postnatal developing retina. At embryonal stages, NGC immunoreactivities were faint. In contrast, at postnatal developmental stages (approximately postnatal day [P]7), intense immunoreactivity was observed in the NFL and IPL, where active dendrite branching was observed, and conventional synapses began to be formed. As retinal layer differentiation proceeded (from P14 to P42), immunoreactivities in the inner retinal layers gradually became fainter. Immunoblot and semiquantitative RT-PCR analyses showed that the peak level of NGC expression occurred on approximately P7 and P14. Glycosylation of the NGC core protein changed as the retinal layers matured. In immunoelectron microscopic analysis, NGC immunoreactivity was located on the axonal membranes of neuronal cells in the postnatal retina, whereas immunoreactivity was reduced on membranes at the adult stage. In retinal ganglion cells in vitro, NGC was highly localized in their spiny budding neurites.
CONCLUSIONS
The results show spatiotemporal expression patterns of NGC, and suggest that it plays a role in the formation of neural networks in retinal development.
影响因子：
4.4
作者：
Y. Otori;Ji-ye Wei;C. Barnstable
通讯作者：
Y. Otori;Ji-ye Wei;C. Barnstable