Safeners improve early‐stage chilling‐stress tolerance in sorghum
Safeners improve early‐stage chilling‐stress tolerance in sorghum
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DOI：
10.1111/jac.12503
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发表时间：
2021-05
影响因子：
3.5
通讯作者：
A. Vennapusa;Y. Assefa;David Sebela;Impa M. Somayanda;R. Perumal;D. Riechers;P. V. Vara Prasad;S. Jagadish
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作者：
A. Vennapusa;Y. Assefa;David Sebela;Impa M. Somayanda;R. Perumal;D. Riechers;P. V. Vara Prasad;S. Jagadish
二色Sorcium bicolor（L.）Moench]在发芽和早期幼苗建立期间对低温（<15°C土壤温度）高度敏感。因此，本研究的主要目的是（a）验证所选高粱基因型的耐冷性的潜在水平，（B）确定用于提高耐冷性的所选安全剂的有效浓度，以及（c）确定与单独的安全剂处理相比，安全剂组合是否进一步提高耐冷性。用5种高粱基因型、4种安全剂（oxabetrinil、fluxofenim、naphthalic anhydride和benoxacor）、9种安全剂浓度（0-25 μM）和2种温度进行了4个实验。在连续15℃下，耐冷基因型的发芽率（98%vs.63%）、根长（3.0vs.0.8cm）和芽长（0.7vs.0.1cm）均高于敏感基因型。在15℃条件下，以最高浓度（20 μ M和25 μM）施用的4种安全剂均能显著促进种子萌发和幼苗生长。四种安全剂的组合在减少低温胁迫引起的伤害方面比单独的、双向或三向组合更有效。在优化遗传背景和农业气候条件对安全剂化学、浓度和种子施用安全剂的适当组合的影响后，增强的耐冷性可以扩展到其他谷类作物。
Sorghum [Sorghum bicolor(L.) Moench] is highly sensitive to chilling temperatures (<15°C soil temperatures) during germination and early seedling establishment. Hence, the main objectives of this research were to (a) validate the potential level of chilling tolerance in selected sorghum genotypes, (b) determine effective concentrations of selected safeners for improving chilling tolerance and (c) determine if safener combinations further enhance chilling tolerance compared to individual safener treatments. Four experiments were conducted with five sorghum genotypes, four safeners (oxabetrinil, fluxofenim, naphthalic anhydride and benoxacor), nine safener concentrations (0–25 μM) and two temperatures. The chilling‐tolerant genotypes recorded higher germination percentage (98% vs. 63%) and root (3.0 vs. 0.8 cm) and shoot lengths (0.7 vs. 0.1 cm) compared to sensitive genotypes under continuous 15℃. Each of the four safeners applied at the highest concentrations tested (20 and 25 μM) significantly enhanced seed germination and seedling growth at 15℃. The combination of four safeners was more effective in reducing chilling stress‐induced injury compared to individual, two‐ or three‐way combinations. Enhanced chilling tolerance may be extended to other cereal crops after optimizing the genetic background and effects of agro‐climatic conditions on safener chemistry, concentration, and proper combination of seed‐applied safeners.