Transplantation of iPSc Ameliorates Neural Remodeling and Reduces Ventricular Arrhythmias in a Post‐Infarcted Swine Model
Transplantation of iPSc Ameliorates Neural Remodeling and Reduces Ventricular Arrhythmias in a Post‐Infarcted Swine Model
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iPSc 移植可改善梗塞后猪模型的神经重塑并减少室性心律失常
DOI：
10.1002/jcb.24687
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发表时间：
2014-03
期刊：
影响因子：
--
通讯作者：
曹克将
中科院分区：
文献类型：
--
作者：
张凤祥;宋桂仙;李小荣;曹克将
Neural remodeling after myocardial infarction (MI) may cause malignant ventricular arrhythmia, which is the main cause of sudden cardiac death following MI. Herein, we aimed to examine whether induced pluripotent stem cells (iPSc) transplantation can ameliorate neural remodeling and reduce ventricular arrhythmias (VA) in a post‐infarcted swine model. Left anterior descending coronary arteries were balloon‐occluded to generate MI. Animals were then divided into Sham, PBS control, and iPS groups. Dynamic electrocardiography programmed electric stimulation were performed to evaluate VA. The spatial distribution of vascularization, Cx43 and autonomic nerve regeneration were evaluated by immunofluorescence staining. Associated protein expression was detected by Western blotting. Likewise, we measured the enzymatic activities of superoxide dismutase and content of malondialdehyde. Six weeks later, the number of blood vessels increased significantly in the iPSc group. The expression of vascular endothelial growth factor and connexin 43 in the iPS group was significantly higher than the PBS group; however, the levels of nerve growth factor and tyrosine hydroxylase were lower. The oxidative stress was ameliorated by iPSc transplantation. Moreover, the number of sympathetic nerves in the iPSc group was reduced, while the parasympathetic nerve fibers had no obvious change. The transplantation of iPSc also significantly decreased the low‐/high‐frequency ratio and arrhythmia score of programmed electric stimulation‐induced VA. In conclusion, iPSc intramyocardial transplantation reduces vulnerability to VAs, and the mechanism was related to the remodeling amelioration of autonomic nerves and gap junctions. Moreover, possible mechanisms of iPSc transplantation in improving neural remodeling may be related to attenuated oxidative stress and inflammatory response. J. Cell. Biochem. 115: 531–539, 2014. © 2013 Wiley Periodicals, Inc.
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DOI：
10.1097/00132586-200306000-00011
发表时间：
2002
期刊：
The New England journal of medicine
影响因子：
--
作者：
M. Goldstein
通讯作者：
M. Goldstein
影响因子：
--
作者：
Chen, Xiang;Gu, Mingbiao;You, Xiaohua
通讯作者：
You, Xiaohua
影响因子：
37.8
作者：
Davani, S;Marandin, A;Kantelip, JP
通讯作者：
Kantelip, JP
影响因子：
2.7
作者：
HASSANKHANI, A;STEINHELPER, ME;FEDEROFF, HJ
通讯作者：
FEDEROFF, HJ
DOI：
10.1016/j.jtcvs.2006.05.034
发表时间：
2006-09-01
影响因子：
6
作者：
Teng, Carolyn J.;Luo, Jun;Shum-Tim, Dominique
通讯作者：
Shum-Tim, Dominique