INVITRO EVALUATION OF THE ANTINEOPLASTIC ACTIVITY OF COMBRETASTATIN-A-4, A NATURAL PRODUCT FROM COMBRETUM-CAFFRUM (ARID SHRUB)
INVITRO EVALUATION OF THE ANTINEOPLASTIC ACTIVITY OF COMBRETASTATIN-A-4, A NATURAL PRODUCT FROM COMBRETUM-CAFFRUM (ARID SHRUB)
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DOI：
10.1097/00001813-199302000-00002
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发表时间：
1993-02-01
影响因子：
2.3
通讯作者：
VONHOFF, DD
中科院分区：
文献类型：
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作者：
ELZAYAT, AAE;DEGEN, D;VONHOFF, DD
Combretastatin A-4是从南非的Combretum caffrum树中分离的天然产物。在本研究中，在放射性测定和人肿瘤克隆试验中检测了考布他汀A-4对8种不同肿瘤细胞系的细胞毒活性，并在人肿瘤克隆试验中检测了其对15种患者肿瘤的细胞毒活性。为了测试考布他汀A-4对肿瘤细胞相对于非肿瘤细胞的优先细胞毒性，还在放射测定法中针对正常人二倍体成纤维细胞和人骨髓细胞进行了测试。在所用的八种细胞系中，考布他汀A-4对其中六种细胞表现出优先的细胞毒性。此外，考布他汀A-4显示出对多种人类肿瘤的浓度依赖性细胞毒性。基于本研究中生成的数据，应在体内临床前模型中进一步测试考布他汀A-4。
Combretastatin A-4 is a natural product which was isolated from the South African tree Combretum caffrum. In this study, the cytotoxic activity of combretastatin A-4 was tested in radiometric and human tumor cloning assays against eight different tumor cell lines and against 15 patient tumors in the human tumor cloning assay. To test the preferential cytotoxicity of combretastatin A-4 against tumor cells versus non-tumor cells, it was also tested in the radiometric assay against both normal human diploid fibroblasts and human bone marrow cells. Of the eight cell lines used, combretastatin A-4 showed preferential cytotoxicity for six of them. In addition, combretastatin A-4 showed a concentration-dependent cytotoxicity against a variety of human tumors. Based on the data generated in this study, combretastatin A-4 should be further tested in in vivo preclinical models.