Rational Design of Organic Probes for Turn-On Two-Photon Excited Fluorescence Imaging and Photodynamic Therapy
Rational Design of Organic Probes for Turn-On Two-Photon Excited Fluorescence Imaging and Photodynamic Therapy
复制标题
用于开启双光子激发荧光成像和光动力治疗的有机探针的合理设计
DOI：
10.1016/j.chempr.2018.12.001
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发表时间：
2019-03-14
期刊：
影响因子：
23.5
通讯作者：
Zhang, Hao-Li
中科院分区：
文献类型：
--
作者：
Sun, Chun-Lin;Li, Jun;Zhang, Hao-Li
在生理条件下，通过温和的刺激可调节双光子吸收（2 PA）截面（delta）值的2 PA有机染料的合理设计仍然是一个挑战。使用模拟辅助结构筛选方法，我们设计了一种缩醛封端的二苯乙烯基苯衍生物（Ace-DSB），它可以很容易地转化为醛封端的分子（Ald-DSB），表现出显着增加在760 nm处的δ值。转化反应可以在细胞内环境中发生，它极大地打开了分子的2 PA吸收能力。此外，Ald-DSB表现出比Ace-DSB高40倍的单重态氧的双光子产生效率。这些开启功能允许增强的双光子激光共聚焦扫描显微成像和用于MCF-7癌细胞和黑色素瘤肿瘤的双光子激发光动力学疗法（2 PE-PDT）。这项工作开辟了一条新的途径，对设计的开启型双光子吸收分子在体外和体内肿瘤成像和2 PA-PDT。
The rational design of two-photon absorption (2PA) organic dyes with 2PA cross-section (delta) values tunable by mild stimuli under physiological conditions remains a challenge. Using a simulation-assisted structure screening method, we have designed an acetal terminated distyrylbenzene derivative (Ace-DSB), which can be readily converted into aldehyde terminated molecules (Ald-DSB) to exhibit a significant increase in delta values at 760 nm. The conversion reaction, which dramatically turns on the 2PA absorption capability of the molecules, can occur in an intracellular environment. Furthermore, Ald-DSB exhibits 40 times higher efficiency of two-photon generation of singlet oxygen than Ace-DSB. These turn-on features allow enhanced two-photon laser confocal scanning microscopic imaging and two-photon excited photodynamic therapy (2PE-PDT) for MCF-7 cancer cells and melanoma tumors. This work opens a new avenue toward the design of turn-on-type two-photon absorption molecules for both in vitro and in vivo tumor imaging and 2PA-PDT.