The caspase 3 sensor Phiphilux G2D2 is activated non-specifically in S1 renal proximal tubules.
The caspase 3 sensor Phiphilux G2D2 is activated non-specifically in S1 renal proximal tubules.
复制标题
Caspase 3 传感器 Phiphilux G2D2 在 S1 肾近曲小管中非特异性激活。
DOI：
10.1080/21659087.2015.1067352
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Dagher,PierreC
中科院分区：
文献类型：
--
作者：
Hato,Takashi;Sandoval,Ruben;Dagher,PierreC
在各种形式的急性肾损伤中，肾小管细胞凋亡是细胞死亡的主要表型。量化固定组织中的细胞凋亡是有问题的，因为细胞凋亡会随着时间的推移而演变，死亡的细胞会被吞噬系统迅速清除。PhiPhilux是一种荧光探针，可被caspase 3特异性激活，并不抑制该效应器caspase的后续活性。它已被成功地用于细胞培养中的细胞凋亡的定量。在这里，我们研究了用活体双光子显微镜测量活体动物肾小管上皮细胞凋亡进展的可行性。我们的结果表明，PhiPhilux可以检测到S2小管的凋亡，但在S1小管中被非特异性地激活。
Tubular cell apoptosis is a major phenotype of cell death in various forms of acute kidney injury. Quantifying apoptosis in fixed tissues is problematic because apoptosis evolves over time and dead cells are rapidly cleared by the phagocytic system. Phiphilux is a fluorescent probe that is activated specifically by caspase 3 and does not inhibit the subsequent activity of this effector caspase. It has been used successfully to quantify apoptosis in cell culture. Here we examined the feasibility of using Phiphilux to measure renal tubular apoptosis progression over time in live animals using intravital 2-photon microscopy. Our results show that Phiphilux can detect apoptosis in S2 tubules but is activated non-specifically in S1 tubules.