Embryonic fibroblast motility and orientation can be influenced by physiological electric fields.
Embryonic fibroblast motility and orientation can be influenced by physiological electric fields.
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DOI：
10.1083/jcb.98.1.296
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发表时间：
1984-01
影响因子：
7.8
通讯作者：
Nuccitelli, R
中科院分区：
文献类型：
--
作者：
Erickson, C A;Nuccitelli, R
众所周知，发育中的胚胎中的上皮层会驱动离子电流通过自身，进而在胚胎内产生小电场。我们假设迁移胚胎细胞的运动可能是由这样的场引导的，并在此报告胚胎鹌鹑体节成纤维细胞运动可以受到小直流电场的强烈影响。这些细胞以三种方式对这些场作出反应：（a）细胞通过沿该方向延伸片状伪足而向场的阴极端迁移。当细胞在血浆中培养时，这种趋电性的阈值场强在 1 至 10 mV/mm 之间。 (b) 细胞的长轴垂直于场线。在场中 90 分钟间隔的该响应的阈值场强在 F12 介质中为 150 mV/mm，在等离子体中为 50 至 100 mV/mm。 (c) 细胞在 400 mV/mm 及以上场强的影响下伸长。因此，这些成纤维细胞能够检测到其宽度上至少低至 0.2 mV 的电压梯度。迄今为止，已在至少一种脊椎动物体内检测到强度比该阈值场大至少 10 倍的电场，因此我们相信这些场效应涵盖了生理范围。
Epithelial layers in developing embryos are known to drive ion currents through themselves that will, in turn, generate small electric fields within the embryo. We hypothesized that the movement of migratory embryonic cells might be guided by such fields, and report here that embryonic quail somite fibroblast motility can be strongly influenced by small DC electric fields. These cells responded to such fields in three ways: (a) The cells migrated towards the cathodal end of the field by extending lamellipodia in that direction. The threshold field strength for this galvanotaxis was between 1 and 10 mV/mm when the cells were cultured in plasma. (b) The cells oriented their long axes perpendicular to the field lines. The threshold field strength for this response for a 90-min interval in the field was 150 mV/mm in F12 medium and between 50 and 100 mV/mm in plasma. (c) The cells elongated under the influence of field strengths of 400 mV/mm and greater. These fibroblasts were therefore able to detect a voltage gradient at least as low as 0.2 mV across their width. Electric fields of at least 10- fold larger in magnitude than this threshold field have been detected in vivo in at least one vertebrate thus far, so we believe that these field effects encompass a physiological range.