Bio-active nanoemulsions enriched with gold nanoparticle, marigold extracts and lipoic acid: In vitro investigations
Bio-active nanoemulsions enriched with gold nanoparticle, marigold extracts and lipoic acid: In vitro investigations
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DOI：
10.1016/j.colsurfb.2014.05.026
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发表时间：
2014-09-01
影响因子：
5.8
通讯作者：
Timur, Suna
中科院分区：
文献类型：
--
作者：
Guler, Emine;Barlas, F. Baris;Timur, Suna
描述了一种制备浓缩草药制剂的新颖有效的方法，并通过体外细胞培养研究研究了它们的潜在应用，包括伤口愈合和抗氧化活性（基于细胞的和无细胞的）。使用纳米乳液系统将金盏花提取物和硫辛酸封端的金纳米颗粒 (AuNP-LA) 浓缩到黑种草油中。这些生物活性化合物的组合用于设计水包油 (O/W) 和油包水 (W/O) 乳液。通过粒度测量来表征所得乳液。通过比色测定和色谱分析检查每种纳米乳液的酚含量。采用两种不同的方法，包括无细胞化学测定（1-二苯基-2-三硝基苯肼法）和基于细胞的抗氧化活性测试来评估抗氧化能力。为了研究草药制剂的生物活性，以Vero细胞系作为模型细胞系，进行了体外细胞培养实验，包括细胞毒性、划痕实验、抗氧化活性和细胞增殖。此外，为了监测应用细胞培养物后纳米乳液的定位，在 FITC 标记后通过荧光显微镜监测细胞图像。所有数据均证实，与未经任何浓缩的苜蓿乳液相比，浓缩的苜蓿制剂表现出更好的抗氧化和伤口愈合活性。总之，将 AuNP-LA 和 Cofficinalis 提取物掺入 N. sativa 乳液中可显着提高生物活性。目前的工作可能支持进一步研究使用其他生物活性剂来富集草药制剂以增强其活性。 (C) 2014 Elsevier B.V. 保留所有权利。
A novel and efficient approach for the preparation of enriched herbal formulations was described and their potential applications including wound healing and antioxidant activity (cell based and cell free) were investigated via in vitro cell culture studies. Nigella sativa oil was enriched with Calendula officinalis extract and lipoic acid capped gold nanoparticles (AuNP-LA) using nanoemulsion systems. The combination of these bio-active compounds was used to design oil in water (O/W) and water in oil (W/O) emulsions. The resulted emulsions were characterized by particle size measurements. The phenolic content of each nanoemulsion was examined by using both colorimetric assay and chromatographic analyses. Two different methods containing cell free chemical assay (1-diphenyl-2-picrylhydrazyl method) and cell based antioxidant activity test were used to evaluate the antioxidant capacities. In order to investigate the bio-activities of the herbal formulations, in vitro cell culture experiments, including cytotoxicity, scratch assay, antioxidant activity and cell proliferation were carried out using Vero cell line as a model cell line. Furthermore, to monitor localization of the nanoemulsions after application of the cell culture, the cell images were monitored via fluorescence microscope after FITC labeling. All data confirmed that the enriched N. sativa formulations exhibited better antioxidant and wound healing activity than N. sativa emulsion without any enrichment. In conclusion, the incorporation of AuNP-LA and C officinalis extract into the N. sativa emulsions significantly increased the bio-activities. The present work may support further studies about using the other bio-active agents for the enrichment of herbal preparations to strengthen their activities. (C) 2014 Elsevier B.V. All rights reserved.