Expression of the D-3 dopamine receptor in peptidergic neurons of the nucleus accumbens: Comparison with the D-1 and D-2 dopamine receptors
Expression of the D-3 dopamine receptor in peptidergic neurons of the nucleus accumbens: Comparison with the D-1 and D-2 dopamine receptors
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DOI：
10.1016/0306-4522(96)00029-2
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发表时间：
1996-07-01
期刊：
影响因子：
3.3
通讯作者：
Bloch, B
中科院分区：
文献类型：
--
作者：
LeMoine, C;Bloch, B
D-3多巴胺受体，属于D-2样受体亚家族，显示出特定的药理学和独特的表达模式，特别是与D-1和D-2受体相比。用[S-35]UTP标记的大鼠D-3互补RNA探针，对大鼠脑内表达D-1受体基因的神经元进行定位。特别是，我们确定的表型的神经元含有D-3信使RNA在脑桥核，相对于纹状体分离的脑啡肽和P物质神经元的人口，通过使用双重原位杂交的策略。此外，与D-1和D-2受体基因表达进行比较，以研究腹侧纹状体中相同神经元内的几种多巴胺受体的潜在共定位。最高水平的D-3信使RNA检测Calleja岛和核的神经元。其他区域，如尾壳核，一些下丘脑，丘脑和皮质区和小脑小叶10也含有低但显着水平的D-3信使RNA。我们的研究结果表明，D-3受体是共同表达的D-1或D-2受体，无论是在核心和外壳区域，在一个亚群的P物质和脑啡肽神经元，分别。这些结果还表明，延髓神经元中有相当一部分只表达D-1或D-2受体，而不表达D-3受体，这意味着延髓核中的多巴胺可能通过两种不同的多巴胺受体的共表达作用于每个传出神经元群体（D-1，+ D-3或D-2 + D-3），并且协同的D-1样/D-2样效应可以在单个神经元的水平上发生，通过D-1和D-3受体在相当大比例的P物质神经元中的共表达（核心和外壳区域分别为16%或26%）。(C)1996年IBRO。出版社：Elsevier Science Ltd
The D-3 dopamine receptor, belonging to the D-2-like receptor subfamily, displays both specific pharmacology and a unique pattern of expression, especially compared to the D-1 and D-2 receptors. A rat D-3 complementary RNA probe labelled with [S-35]UTP was used to map the neurons expressing the D-1 receptor gene in the rat brain. In particular, we identified the phenotype of the neurons containing D-3 messenger RNA in the nucleus accumbens, with respect to the striatal segregated populations of enkephalin and substance P neurons, by using strategies of double in situ hybridization. In addition, comparison with D-1 and D-2 receptor gene expression was performed to study the potential co-localization of several dopamine receptors within the same neurons in the ventral striatum. The highest level of D-3 messenger RNA was detected in the islands of Calleja and the nucleus accumbens. Other areas such as the caudate-putamen, some hypothalamic, thalamic and cortical areas and lobule 10 of the cerebellum also contained low but significant levels of D-3 messenger RNA. Our results demonstrate that the D-3 receptor is co-expressed either with D-1 or with D-2 receptor, both in the core and shell regions, in a subpopulation of substance P and enkephalin neurons, respectively. They also suggest that a significant part of the accumbal neurons only express either D-1 or D-2, without co-expression with the D-3 receptor.These data imply that dopamine in the nucleus accumbens may act on each population of efferent neurons via the co-expression of two distinct dopamine receptors (D-1, + D-3 or D-2 + D-3), and that synergistic D-1-like/D-2-like effects may occur al the level of a single neuron, through the co-expression of D-1 and D-3 receptors in a significant proportion of substance P neurons (16% or 26% in the core and shell regions, respectively). (C) 1996 IBRO. Published by Elsevier Science Ltd.