Growth of Artemisia annua hairy roots in liquid- and gas-phase reactors
Growth of Artemisia annua hairy roots in liquid- and gas-phase reactors
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DOI：
10.1002/bit.10389
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发表时间：
2002-11-20
影响因子：
3.8
通讯作者：
Wyslouzil, BE
中科院分区：
文献类型：
--
作者：
Kim, YJ;Weathers, PJ;Wyslouzil, BE
在液相鼓泡塔和气相营养雾化反应器中培养青蒿毛状根。在大多数情况下，鼓泡塔反应器比雾化反应器积累了更多的生物量；最终观察到的最高生物量浓度鼓泡塔反应器为15.3gDW/L，雾化反应器为14.4gDW/L。进一步的分析表明，在雾化模式开始时，雾化反应器中的平均比生长速率基本上是恒定的，并且与生物量浓度无关。相反，在低填充密度下，鼓泡塔反应器中的平均增长速率高于雾化反应器中的平均增长速率，在高填充密度下下降到可比的速率。最后，气溶胶沉积模型被用来比较雾化反应器中根床捕获的介质体积与保持特定生长速率所需的介质体积。结果表明，在目前的运行条件下，雾化反应器中的生长速率较低可能是由于养分供应不足所致。(C)2002年威利期刊公司。
Artemisia annua hairy roots were grown in liquid-phase bubble column and gas-phase nutrient mist reactors. In most cases the bubble column reactor accumulated more biomass than the mist reactor; the highest final biomass concentrations observed were 15.3 g DW/L in the bubble column reactor and 14.4 g DW/L in the mist reactor. Further analysis showed that the average specific growth rate in the mist reactors was essentially constant and independent of the biomass concentration at the beginning of the mist mode. In contrast, at low packing densities the average growth rate in the bubble column reactors was higher than in the mist reactors, decreasing to comparable rates at high packing densities. Finally, an aerosol deposition model was used to compare the volume of medium captured by the root bed in the mist reactor to the volume of medium required to maintain a specified growth rate. The results suggest that under the current operating conditions, lower growth rates in the mist reactor may be due to insufficient nutrient availability. (C) 2002 Wiley Periodicals, Inc.