The Phytophthora effector Avh94 manipulates host jasmonic acid signaling to promote infection
The Phytophthora effector Avh94 manipulates host jasmonic acid signaling to promote infection
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DOI：
10.1111/jipb.13358
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发表时间：
2022-10-14
影响因子：
11.4
通讯作者：
Wang，Yuanchao
中科院分区：
文献类型：
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作者：
Zhao，Yao;Yang，Bo;Wang，Yuanchao
大豆疫霉（Phytophthora sojae）是大豆根腐病的病原菌。在大豆定殖后，大豆疫霉分泌各种RXLR效应物以抑制宿主免疫应答，支持成功感染。先前的研究表明，RXLR效应子Avh 94作为毒力效应子发挥作用，但其在毒力中作用的分子机制仍不清楚。在这里，我们证明了Avh 94在植物和病原体中的过表达促进了植物病原体的感染。Avh 94与大豆JAZ 1/2相互作用，JAZ 1/2是茉莉酸（JA）信号传导的阻遏物。Avh 94稳定JAZ 1/2以抑制JA信号传导，JAZ 1/2的沉默增强大豆对P. sojae此外，过表达Avh 94的大豆假单胞菌抑制JA信号传导。此外，外源茉莉酸甲酯的施用提高了植物对疫霉的抗性。综上所述，这些发现表明P. sojae利用RXLR效应子劫持JA信号传导，从而促进感染。
The oomycete pathogenPhytophthora sojaeis a causal agent of soybean root rot. Upon colonization of soybeans,P. sojaesecretes various RXLR effectors to suppress host immune responses, supporting successful infection. Previous research has demonstrated that the RXLR effector Avh94 functions as a virulence effector, but the molecular mechanism underlying its role in virulence remains unknown. Here, we demonstrate that Avh94 overexpression in plants and pathogens promotesPhytophthorainfection. Avh94 interacts with soybean JAZ1/2, which is a repressor of jasmonic acid (JA) signaling. Avh94 stabilizes JAZ1/2 to inhibit JA signaling and silencing ofJAZ1/2enhances soybean resistance againstP. sojae. Moreover,P. sojaelines overexpressingAvh94inhibit JA signaling. Furthermore, exogenous application of methyl jasmonate improves plant resistance toPhytophthora. Taken together, these findings suggest thatP. sojaeemploys an RXLR effector to hijack JA signaling and thereby promote infection.