Community structure and functional diversity of epiphytic bacteria and planktonic bacteria on submerged macrophytes in Caohai lake, Southwest of China
Community structure and functional diversity of epiphytic bacteria and planktonic bacteria on submerged macrophytes in Caohai lake, Southwest of China
复制标题
草海湖沉水植物附生细菌和浮游细菌的群落结构和功能多样性
DOI：
10.1007/s13213-019-01485-4
复制
发表时间：
2019
影响因子：
3
通讯作者：
Haipeng Cao
中科院分区：
文献类型：
--
作者：
Dingbo Yan;Pinhua Xia;Xu Song;Tao Lin;Haipeng Cao
目的沉水植物表面的附生细菌在湖泊生物多样性和生态过程中起着重要作用。然而，与附生细菌相比，人们对附生细菌的群落结构和功能的了解还很少。我们利用16 S rRNA基因高通量测序和功能预测分析，探讨了典型沉水植物附生细菌和浮游细菌的结构和功能多样性结果草海浮游细菌与附生细菌的种类组成相似，门占88.89%，两种群落共有的属占总属数的77.21%，共有的OTU占总属数的65.78%，变形菌和拟杆菌是两种群落共有的优势类群。然而，也有一些特殊的类群。此外，附生细菌群落表现出显着不同的结构，从那些在水中，和丰富的OTU有相反的组成。水生环境参数对浮游细菌群落的解释比例显著高于附生细菌，表明附生生物膜的生境微环境可能是附生细菌群落的强大驱动力。功能预测分析（Functional Annotation of Prosthetic Taxa，FAPROTAX）发现，附生细菌和反硝化细菌以异养功能为主，但附生细菌具有更突出的发酵和反硝化功能（硝酸盐还原，硝酸盐呼吸，和亚硝酸盐呼吸）结论本研究加深了对沉水植物附生细菌群落结构和功能的认识叶，其在湖泊反硝化中的作用不容忽视。
PurposeEpiphytic bacteria on the surfaces of submerged macrophytes play an important role in lake biodiversity and ecological processes. However, compared with planktonic bacteria, there is poor understanding of the community structure and function of epiphytic bacteria.MethodsHere, we used 16S rRNA gene high-throughput sequencing and functional prediction analysis to explore the structural and functional diversity of epiphytic bacteria and planktonic bacteria of a typical submerged macrophyte (Potamogeton lucens) in Caohai Lake.ResultsThe results showed that the species composition of epiphytic and planktonic bacteria was highly similar as 88.89% phyla, 77.21% genera and 65.78% OTUs were shared by the two kinds of samples.ProteobacteriaandBacteroideteswere dominant phyla shared by the two kinds of communities. However, there are also some special taxa. Furthermore, the epiphytic bacterial communities exhibited significantly different structures from those in water, and the abundant OTUs had opposite constituents. The explained proportion of the planktonic bacterial community by aquatic environmental parameters is significantly higher than that of epiphytic bacteria, implying that the habitat microenvironment of epiphytic biofilms may be a strong driving force of the epiphytic bacterial community. Functional predictive analysis (Functional Annotation of Prokaryotic Taxa, FAPROTAX) found that epiphytic bacteria and planktonic bacteria are dominated by heterotrophic functions, but epiphytic bacteria have more prominent fermentation and denitrification functions (nitrate reduction, nitrate respiration, and nitrite respiration) than planktonic bacteria.ConclusionThis study has increased our understanding of the communities and functions of epiphytic bacteria on submerged macrophyte leaves, and their role in lake denitrification cannot be ignored.