Acutely isolated astrocytes as models to probe astrocyte functions
Acutely isolated astrocytes as models to probe astrocyte functions
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DOI：
10.1016/s0197-0186(99)00144-8
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发表时间：
2000-04-01
影响因子：
4.2
通讯作者：
Zhou, M
中科院分区：
文献类型：
--
作者：
Kimelberg, HK;Cai, Z;Zhou, M
神经科学家越来越意识到并接受星形胶质细胞在CNS中可能具有许多重要功能的概念。在建立这些功能的一个限制是什么构成合适的实验方法的常见问题。星形胶质细胞功能研究的一个主要实验步骤是广泛使用原代星形胶质细胞培养，这是Leif Hertz开创的一种方法。然而，现在越来越清楚的是，在这项工作的基础上，现在需要进行实验性的范式转变。也就是说，越来越多地研究与原位条件相对应的制剂，例如切片。另一种实验系统是新鲜分离的星形胶质细胞，其中细胞具有原代星形胶质细胞培养物的一些技术优势。我们实验室最近的实验表明，这些细胞表达代谢型谷氨酸受体。给出的例子是如何功能受体的研究和通道活性测量膜片钳电生理学可以与单细胞RT-PCR相结合，以进一步定义的受体或通道类型描述，以说明这种制剂的用途。(C)2000爱思唯尔科技有限公司版权所有。
Neuroscientists have become increasingly aware and accepting of the concept that astrocytes likely have many important functions in the CNS. One limitation in establishing these functions is the usual problem of what constitutes suitable experimental approaches. A major experimental step for functional studies of astrocytes has been the widespread use of primary astrocyte cultures, an approach that Leif Hertz pioneered. However, it is now becoming clear that, building on this work, an experimental paradigm shift is now needed. Namely, to increasingly study preparations corresponding to in situ conditions, such as slices. An alternative experimental system where the cells have some of the technical advantages of primary astrocyte cultures is freshly isolated astrocytes. Recent experiments from our laboratory have shown metabotropic glutamate receptor expression by such cells. Examples are given of how functional receptor studies and channel activity measured by patch clamp electrophysiology can be combined with single cell RT-PCR to define further the receptor or channel type are described to illustrate the uses of such preparations. (C) 2000 Elsevier Science Ltd. All rights reserved.