Independent evaluation of the Nigrosin-Eosin modification of the Kato-Katz technique
Independent evaluation of the Nigrosin-Eosin modification of the Kato-Katz technique
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DOI：
10.1046/j.1365-3156.1999.00356.x
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发表时间：
1999-01-01
影响因子：
3.3
通讯作者：
Savioli, L
中科院分区：
文献类型：
--
作者：
Ramsan, M;Montresor, A;Savioli, L
一种新的改良定量加藤-卡茨厚涂片技术检测粪便中的蠕虫卵保存钩虫卵长时间不变，而与经典的加藤-卡茨技术，他们消失后约2小时在热带气候，因此必须在样品采集后数小时内读取幻灯片。为了对这两种实验室技术进行独立比较，在两次调查中对263名学童的粪便涂片进行了检查，并计算了患病率、感染强度和调查费用。两种方法对不同强度等级的样本的检出率和分层率均无统计学差异，对T. trichiura和A.钩虫平均感染强度差异有高度显著性（P <0.001）。从公共卫生的角度来看，这两种方法提供了类似的结果，但由于其简单和广泛使用的经典Kato-Katz技术仍然是首选的社区调查的土壤传播线虫。然而，黑色素-曙红方法具有几个优点，在某些情况下可能是一种有价值的替代方法。
A new modified quantitative Kato-Katz thick-smear technique for the detection of helminth eggs in faeces preserves hookworm eggs unaltered for a long time, while with the classic Kato-Katz technique, they disappear after approximate to 2 h in tropical climates and thus slides must be read within hours after sample collection. For an independent comparison of these two laboratory techniques, faecal smears from 263 school children were examined in two surveys and prevalence, intensity of infection and costs of surveys calculated. There was no statistical difference between the methods in detecting prevalence and stratification of the sample in different classes of intensity While there was no statistical difference for the arithmetic mean of the epg for T. trichiura and only a small difference for A. lumbricoides (P = 0.04), we observed a highly significant difference for hookworm mean intensities of infections (P < 0.001). From the public health viewpoint both methods provided similar results, but due to its simplicity and widespread use the classical Kato-Katz technique remains first choice for community investigation of soil-transmitted nematodes. However, the Nigrosin-Eosin approach has several advantages and can be a valuable alternative in certain circumstances.