ASSESSMENT OF CELLULAR VIABILITY IN CARDIOVASCULAR TISSUE AS STUDIED WITH 3H-PROLINE AND 3H-INULIN
ASSESSMENT OF CELLULAR VIABILITY IN CARDIOVASCULAR TISSUE AS STUDIED WITH 3H-PROLINE AND 3H-INULIN
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DOI：
10.1093/cvr/24.7.528
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发表时间：
1990-07-01
影响因子：
10.8
通讯作者：
WOLFINBARGER, L
中科院分区：
文献类型：
--
作者：
HU, JF;GILMER, L;WOLFINBARGER, L
本研究的目的是开发一种用于评估心血管组织中细胞活力的可定量测定方法。放射性标记的脯氨酸和菊粉被用来评估从猪心脏瓣膜的瓣尖组织的细胞组分的代谢活力。在死亡后20分钟内取出猪心，并在组织培养基中冰上运输。快速解剖瓣尖组织，并将其置于生理培养基中的冰上直至进行分析。放射性标记的菊粉被证明是一个有用的标志物，用于确定细胞外组织体积中存在的放射性标记的脯氨酸的量，允许计算细胞组分积累的放射性标记的脯氨酸的量。脯氨酸积累的细胞成分的脯氨酸浓度，时间允许脯氨酸积累，在体外冷缺血，代谢毒物的影响。基于mg组织蛋白，主动脉瓣、肺动脉瓣、二尖瓣和三尖瓣组织的脯氨酸和菊粉蓄积相当，表明这些瓣膜组织可互换用于评估心血管组织细胞组分的代谢活力。放射性标记的脯氨酸和菊粉转运测定允许总细胞活力的定量估计。
The aim of the study was to develop a quantifiable assay for the assessment of cellular viability in cardiovascular tissue. Radiolabelled proline and inulin were used to assess metabolic viability of the cellular component of cuspal tissue from porcine heart valves. Pig hearts were removed within 20 min of death and transported on ice in tissue culture medium. Cuspal tissues were dissected rapidly and held on ice in physiological medium until assayed. Radiolabelled inulin was shown to be a useful marker for determining the amount of radiolabelled proline present in the extracellular tissue volume, permitting calculation of the amount of radiolabelled proline accumulated by the cellular component. Proline accumulation by the cellular component was affected by concentration of proline, time allowed for proline accumulation, in vitro cold ischaemia, and metabolic poisons. Based on mg tissue protein, proline and inulin accumulations were equivalent for aortic, pulmonary, mitral, and tricuspid valve tissues, suggesting that these valve tissues may be used interchangeably in assessment of metabolic viability of cellular components of cardiovascular tissue. Radiolabelled proline and inulin transport assays allow a quantitative estimate of total cellular viability.