Efficient biocatalyst by encapsulating lipase into nanoporous gold.
Efficient biocatalyst by encapsulating lipase into nanoporous gold.
复制标题
将脂肪酶封装到纳米孔金中的高效生物催化剂
DOI：
10.1186/1556-276x-8-180
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发表时间：
2013-04-19
影响因子：
--
通讯作者：
Xu P
中科院分区：
文献类型：
--
作者：
Du X;Liu X;Li Y;Wu C;Wang X;Xu P
脂肪酶是生物技术应用中最重要的生物催化剂之一。固定化是提高脂肪酶稳定性和重复使用性的有效方法。本研究以一种具有可调孔隙率和良好生物相容性的新型纳米多孔材料-纳米多孔金（NPG）作为固定化脂肪酶的有效载体。NPG的孔径大小和吸附时间对脂肪酶-NPG生物复合材料的构建起关键作用。NPG的形态和组成之前和之后的脂肪酶加载验证使用扫描电子显微镜，配备有能量色散X射线光谱仪。所得脂肪酶-NPG生物复合物具有良好的催化活性和显著的可重复使用性。孔径为35 nm的脂肪酶-NPG生物复合物在10次循环后的催化活性没有下降。此外，与游离脂肪酶相比，脂肪酶-NPG复合物在更宽的pH范围和更高的温度下都表现出较高的催化活性。此外，通过匹配蛋白质的直径和孔径，可以防止脂肪酶从NPG中的浸出。因此，将酶包封在NPG中对于建立新的功能是非常有用的，并且将在不同的化学过程中具有广泛的应用。
Lipases are one of the most important biocatalysts for biotechnological applications. Immobilization is an efficient method to increase the stability and reusability of lipases. In this study, nanoporous gold (NPG), a new kind of nanoporous material with tunable porosity and excellent biocompatibility, was employed as an effective support for lipase immobilization. The pore size of NPG and adsorption time played key roles in the construction of lipase-NPG biocomposites. The morphology and composition of NPG before and after lipase loading are verified using a scanning electron microscope, equipped with an energy-dispersive X-ray spectrometer. The resulting lipase-NPG biocomposites exhibited excellent catalytic activity and remarkable reusability. The catalytic activity of the lipase-NPG biocomposite with a pore size of 35 nm had no decrease after ten recycles. Besides, the lipase-NPG biocomposite exhibited high catalytic activity in a broader pH range and higher temperature than that of free lipase. In addition, the leaching of lipase from NPG could be prevented by matching the protein’s diameter and pore size. Thus, the encapsulation of enzymes within NPG is quite useful for establishing new functions and will have wide applications for different chemical processes.