Bleomycin-induced apoptosis of alveolar epithelial cells requires angiotensin synthesis de novo
Bleomycin-induced apoptosis of alveolar epithelial cells requires angiotensin synthesis de novo
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DOI：
10.1152/ajplung.00273.2002
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发表时间：
2003-03-01
影响因子：
4.9
通讯作者：
Uhal, BD
中科院分区：
文献类型：
--
作者：
Li, XP;Zhang, HY;Uhal, BD
原代培养的大鼠肺泡上皮细胞(AECs)或人肺泡上皮细胞(A549)在博莱霉素(Bleo)作用下，表现出浓度依赖性的凋亡，表现为细胞核形态改变、DNA断裂、caspase-3激活和随时间推移的细胞净丢失。在这两种细胞培养模型中，暴露于Bleo会导致血管紧张素原(Angen)mRNA的时间依赖性增加。Ang基因反义寡核苷酸对Bleo诱导的大鼠AEC和A549细胞凋亡的抑制率分别为83%和84%(P
Primary cultures of rat type II alveolar epithelial cells (AECs) or human AEC-derived A549 cells, when exposed to bleomycin (Bleo), exhibited concentration-dependent apoptosis detected by altered nuclear morphology, fragmentation of DNA, activation of caspase-3, and net cell loss over time. In both cell culture models, exposure to Bleo caused time-dependent increases in angiotensinogen (ANGEN) mRNA. Antisense oligonucleotides against ANGEN mRNA inhibited Bleo-induced apoptosis of rat AEC or A549 cells by 83 and 84%, respectively (P