microRNA-195 inhibits the proliferation, migration and invasion of cervical cancer cells via the inhibition of CCND2 and MYB expression
microRNA-195 inhibits the proliferation, migration and invasion of cervical cancer cells via the inhibition of CCND2 and MYB expression
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DOI：
10.3892/ol.2015.3541
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发表时间：
2015-10-01
期刊：
影响因子：
2.9
通讯作者：
Jiang, Jie
中科院分区：
文献类型：
--
作者：
Du, Xinjie;Lin, Li;Jiang, Jie
microRNAs （miRNA/miR）在宫颈癌发展中的作用在很大程度上仍不清楚。本研究探讨了miR-195在宫颈病变中的作用。癌症的发展。miR-195模拟物在宫颈癌He La细胞系中的表达显著降低了细胞体外增殖、迁移和侵袭能力。利用miRNA靶标预测算法和报告基因检测，细胞周期蛋白D2 （CCND2）和v-myb禽成髓细胞病病毒致癌基因同源物（MYB）被确定为miR-195的直接靶标。此外，miR-195在mRNA和蛋白水平上抑制了HeLa细胞中CCND2和MYB的表达。最后，与正常组织相比，miR-195在宫颈癌组织中的表达下调。总之，这些数据表明miR-195在宫颈癌中是一种肿瘤抑制因子。
The functions of microRNAs (miRNA/miR) in the development of cervical cancer remain largely undefined. The present study investigated the role of miR-195 in cervical. cancer development. The expression of miR-195 mimics in the cervical cancer He La cell line significantly decreased the cell proliferation, migration and invasion capacities in vitro. Using miRNA target prediction algorithms and reporter assays, cyclin D2 (CCND2) and v-myb avian myeloblastosis viral oncogene homolog (MYB) were identified as direct targets of miR-195. Moreover, miR-195 repressed the expression of CCND2 and MYB in the HeLa cells at the mRNA and protein levels. Finally, the expression of miR-195 was downregulated in cervical cancer tissues compared with normal tissues. Together, these data suggest that miR-195 is a tumor suppressor in cervical cancer.