Accurate prediction of anti‐phagocytic activity of Vibrio vulnificus by measurement of bacterial adherence to hydrocarbons. Prediction of Anti‐Phagocytic Activity
Accurate prediction of anti‐phagocytic activity of Vibrio vulnificus by measurement of bacterial adherence to hydrocarbons. Prediction of Anti‐Phagocytic Activity
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通过测量细菌对碳氢化合物的粘附来准确预测创伤弧菌的抗吞噬活性。
DOI：
10.1111/apm.12910
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
and Shunji Ueno
中科院分区：
文献类型：
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作者：
Takehiro Kado;Takashige Kashimoto*;Kohei Yamazaki;Kaho Matsuda;and Shunji Ueno
创伤弧菌可通过暴露于开放的伤口引起坏死性软组织感染，而伤口感染的潜伏期仅为16小时左右。这些事实强烈表明，逃避先天免疫细胞吞噬的机制是其致病性的关键。疏水相互作用是细菌与吞噬细胞的结合机制之一。维持细胞表面疏水性（CSH）的几个因素可以促进抗吞噬活性。在这项研究中，我们试图识别v。创伤基因参与维持CSH，以阐明抗吞噬活性的机制。我们使用标记转座子诱变（STM）获得了143个在宿主中失去增殖能力的突变体。这些突变体的CSH是通过细菌粘附碳氢化合物（BATH）测定法测定的。只有4个突变体的CSH与野生型（WT）有显著差异。在这四种突变体中，degSmutant （degS::Tn）在抗吞噬作用实验中表现出比WT更低的抗吞噬活性，尽管degS::Tn显示出不透明型菌落。此外，皮下接种的小鼠存活时间延长。这些事实表明，巴斯法是一种更适合分析v抗吞噬活性的方法。创伤菌菌落形态的比较。
Vibrio vulnificuscan cause necrotizing soft tissue infection via exposure through an open wound, and the incubation period in cases of wound infection is only about 16 h. These facts strongly suggest that mechanisms to evade innate immune cell phagocytosis are essential for its pathogenicity. Hydrophobic interaction is one of the binding mechanisms between bacteria and phagocytes. Several factors that maintain cell surface hydrophobicity (CSH) can contribute to anti‐phagocytic activity. In this study, we tried to identifyV. vulnificusgenes involved in maintaining the CSH, in order to elucidate mechanisms of anti‐phagocytic activity. We obtained 143 mutants that had lost their ability to proliferate in the host, using signature‐tagged transposon basis mutagenesis (STM). The CSH of these mutants was measured by the bacterial adherence to hydrocarbons (BATH) assay. The CSH of only four mutants differed significantly from that of wild type (WT). Of these four mutants,degSmutant (degS::Tn) showed lesser anti‐phagocytic activity than WT in the opsonophagocytosis assay, even thoughdegS::Tn showed opaque‐type colonies. Furthermore, survival times of mice subcutaneously inoculated withdegS::Tn were prolonged. These facts indicated that the BATH assay is a more suitable method of analyzing the anti‐phagocytic activity ofV. vulnificusthan the comparison of colony morphology.