GENETIC DIVERSITY IN HARD RED SPRING WHEAT-BASED ON SEQUENCE-TAGGED-SITE PCR MARKERS
GENETIC DIVERSITY IN HARD RED SPRING WHEAT-BASED ON SEQUENCE-TAGGED-SITE PCR MARKERS
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DOI：
10.2135/cropsci1994.0011183x003400060037x
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发表时间：
1994-11-01
期刊：
影响因子：
2.3
通讯作者：
TALBERT, LE
中科院分区：
文献类型：
--
作者：
CHEN, HB;MARTIN, JM;TALBERT, LE
小麦（Triticum aestivum L.）亲本间的遗传变异是通过杂交和选择获得优良后代的必要条件。然而，杂交通常在具有相似农艺和最终用途特征的优良品系之间进行。因此，任何一类小麦的种质基础都有可能出现不希望出现的变窄。通过与代表一系列类型和地理来源的小麦样本进行比较，确定了硬红春小麦的相对遗传多样性。采用聚合酶链反应方法，利用38个序列标记引物对三组材料的DNA多态性频率进行分析。组I包括蒙大拿和北达科他州生产的10个优质硬红春小麦品种，组II包括来自北美大平原的15个硬红春小麦品种和品系，组III包括20个品种，代表了广泛的收集和形态类型。38组引物中有24组（63%）和76组引物-酶组合中有31组（41%）存在多态性。45个品系间两两比较的遗传相似度范围为0.65 ~ 0.99。平均遗传相似度为0.81。硬红春小麦的遗传相似性为0.88，而基础广泛的III类群的遗传相似性为0.78。结果表明，相对于六倍体小麦类间和类内多样性水平而言，六倍体硬红春小麦的选育池较窄。
Genetic variation among wheat (Triticum aestivum L.) parents is necessary to derive superior progeny from crossing and selection. However, crosses are often performed among elite lines with similar agronomic and end-use characteristics. Thus, the potential exists for an undesirable narrowing of the germplasm base for any particular class of wheat. The relative genetic diversity within hard red spring wheat was determined in comparison to a sample of wheat accessions representing an array of types and geographic origins. Three groups of accessions were assayed for the frequencies of DNA polymorphism using a total of 38 sequence-tagged-she primer sets with polymerase chain reaction. Group I contained 10 elite hard red spring wheat cultivars under production in Montana and North Dakota, Group II included 15 hard red spring wheat cultivars and lines from the North American Great Plains, and Group III contained 20 accessions representing a wide range of collection and morphological types. Twenty-four of 38 primer sets (63%) and 31 of 76 primer-enzyme combinations (41%) revealed polymorphisms. The range of genetic similarity estimated by percentage of shared restriction fragments varied from 0.65 to 0.99 among all pairwise comparisons among the 45 lines. Average genetic similarity was 0.81. Genetic similarity among the hard red spring wheats was 0.88, whereas genetic similarity among the broadly based Group III was 0.78. Our results showed that the breeding pool for hexaploid hard red spring wheat is narrow relative to levels of diversity among and within classes in hexaploid wheat.