RNA Interference to Knock Down Gene Expression.
RNA Interference to Knock Down Gene Expression.
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DOI：
10.1007/978-1-4939-7471-9_16
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Han H
中科院分区：
文献类型：
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作者：
Han H
RNA 干扰 (RNAi) 是双链 RNA (dsRNA) 通过靶向 mRNA 降解来诱导序列特异性基因沉默的生物过程。作为一种在转录后敲低单个基因表达的工具，RNAi 已被广泛用于研究基因的细胞功能。在本章中，我将描述使用基因特异性、合成的短干扰 RNA (siRNA) 在哺乳动物细胞中诱导基因沉默的程序。提供了使用基于脂质的转染试剂和电穿孔技术的方案。还讨论了与 siRNA 技术相关的潜在挑战和问题。
RNA interference (RNAi) is a biological process by which double-stranded RNA (dsRNA) induces sequence-specific gene silencing by targeting mRNA for degradation. As a tool for knocking down the expression of individual genes post transcriptionally, RNAi has been widely used to study the cellular function of genes. In this chapter, I describe procedures for using gene-specific, synthetic, short interfering RNA (siRNA) to induce gene silencing in mammalian cells. Protocols for using lipid-based transfection reagents and electroporation techniques are provided. Potential challenges and problems associated with the siRNA technology are also discussed.