Serum levels of microRNAs in patients with heart failure
Serum levels of microRNAs in patients with heart failure
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DOI：
10.1093/eurjhf/hfr155
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发表时间：
2012-02-01
影响因子：
18.2
通讯作者：
Amir, Offer
中科院分区：
文献类型：
--
作者：
Goren, Yaron;Kushnir, Michal;Amir, Offer
目的心衰患者的诊断和风险分层仍然是一个挑战。被称为microrna的小非编码rna调节基因表达，似乎在心力衰竭的发病机制中发挥重要作用。在目前的研究中，我们的目的是表征慢性收缩期心力衰竭患者与对照组血清中microRNAs的水平，并评估特定microRNAs水平升高与心力衰竭患者临床预后参数之间可能的相关性。方法与结果采用定量逆转录聚合酶链反应（qRT-PCR）技术检测了30例稳定型慢性收缩期心力衰竭患者和30例对照组血清中186种microrna的水平。描述了两组之间microRNA水平的差异，并根据心力衰竭组中升高最显著的四种特定microRNA （miR-423-5p, miR-320a， miR-22和miR-92b）的水平定义了评分。该评分用于区分心力衰竭患者和对照组，灵敏度和特异性为90%。此外，在心力衰竭组中，评分与血清利钠肽水平升高、QRS宽、左心室和左心房扩张等重要临床预后参数之间存在显著相关性（r = 0.63, P = 3e-4; P = 0.009; P = 0.03; P = 0.01）。结论血清中miR-423-5p、miR-320a、miR-22和miR-92b特异性microrna水平升高可识别收缩期心力衰竭患者，并与重要的临床预后参数相关。
Aims Diagnosis and risk stratification of patients with heart failure remain a challenge. The small non-coding RNAs known as microRNAs regulate gene expression and seem to play an important role in the pathogenesis of heart failure. In the current study, we aim to characterize the levels of microRNAs in the sera of chronic systolic heart failure patients vs. controls and assess the possible correlation between elevation in the levels of specific microRNAs and clinical prognostic parameters in heart failure patients.Methods and results The levels of 186 microRNAs were measured in the sera of 30 stable chronic systolic heart failure patients and 30 controls using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The differences in microRNA levels between the two groups were characterized, and a score, based on the levels of four specific microRNAs with the most significant increase in the heart failure group (miR-423-5p, miR-320a, miR-22, and miR-92b), was defined. The score was used to discriminate heart failure patients from controls with a sensitivity and specificity of 90%. Moreover, in the heart failure group, there was a significant association between the score and important clinical prognostic parameters such as elevated serum natriuretic peptide levels, a wide QRS, and dilatation of the left ventricle and left atrium (r = 0.63, P = 3e-4; P = 0.009; P = 0.03; and P = 0.01, respectively).Conclusions Elevated serum levels of specific microRNAs: miR-423-5p, miR-320a, miR-22, and miR-92b, identify systolic heart failure patients and correlate with important clinical prognostic parameters.