Expression of natriuretic peptide-activated guanylate cyclases by cholinergic and dopaminergic amacrine cells of the rat retina
Expression of natriuretic peptide-activated guanylate cyclases by cholinergic and dopaminergic amacrine cells of the rat retina
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DOI：
10.1016/j.peptides.2007.11.021
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发表时间：
2008-04-01
期刊：
影响因子：
3
通讯作者：
Tooyama, Ikuo
中科院分区：
文献类型：
--
作者：
Abdelalim, Essam Mohamed;Masuda, Chiaki;Tooyama, Ikuo
最近，在视网膜的胆碱能和多巴胺能无长突细胞中检测到利钠肽。我们对大鼠视网膜切片进行免疫荧光标记，以检测钠尿肽激活的鸟苷环化酶(NPR-A和NPR-B)在大鼠视网膜中的免疫反应性，特别是它们是否定位于多巴胺能和胆碱能无长突细胞。NPR-A和NPR-B在包括无长突细胞在内的多层视网膜中均有表达。在无长突细胞中，NPRA和NPR-B与多巴胺能细胞的标志物酪氨酸羟化酶共定位。NPR-B，而不是NPR-A，定位于表达胆碱能细胞标志物胆碱乙酰转移酶(ChAT)的无长突细胞。这些发现表明，利钠肽在大鼠视网膜多巴胺能和胆碱能无长突细胞中具有不同的调节系统。(C)2008 Elsevier Inc.保留所有权利。
Recently, the natriuretic peptides were detected in the cholinergic and dopaminergic amacrine cells of the retina. We performed immunofluorescence labeling of rat retinal sections to examine the immunoreactivity of natriuretic peptide-activated guanylate cyclases (NPR-A and NPR-B) in the rat retina, in particular whether they were localized to dopaminergic and cholinergic amacrine cells. NPR-A and NPR-B immunoreactivity was detected in several layers of the retina including amacrine cells. In amacrine cells, both NPRA and NPR-B were co-localized with tyrosine hydroxylase, a marker of dopaminergic cells. NPR-B, but not NPR-A, was localized to amacrine cells expressing choline acetyltransferase (ChAT), a marker of cholinergic cells. These findings suggest that natriuretic peptides have different regulatory systems in dopaminergic and cholinergic amacrine cells in rat retina. (C) 2008 Elsevier Inc. All rights reserved.