Interaction of the antitumor antibiotic mitomycin C with Z-DNA.
Interaction of the antitumor antibiotic mitomycin C with Z-DNA.
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抗肿瘤抗生素丝裂霉素 C 与 Z-DNA 的相互作用。
DOI：
10.1080/07391102.1988.10506500
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发表时间：
1988
影响因子：
4.4
通讯作者：
Tomasz,M
中科院分区：
文献类型：
--
作者：
Chawla,AK;Tomasz,M
Mitomycin C (MC), an antitumor antibiotic, alkylated Z-DNAs such as poly(dG-dC)/CO(NH3)3+6, poly(dG-m5dC)/Mg2+and brominated poly(dG-dC) upon reductive activation. Computer-generated energy-minimized molecular models indicated that monofunctional alkylation of Z-DNA at the N2-position of guanine by MC did not distort Z-DNA geometry, but bifunctional alkylation, leading to interstrand crosslinks between two N2-positions of guanine was sterically unfavorable. The above three Z-DNA's were exposed both to mono-functionally and bifunctionally activated MC in separate experiments and the resulting covalent MC-polynucleotide complexes were examined for conformation and for covalent MC-adducts, by circular dichroism (CD) spectroscopy and HPLC analysis of nuclease digests, respectively. Monofunctionally activated MC alkylated all three polynucleotides in their Z-forms, resulting in the same monofunctional N2-guanine adduct as that known to be formed with B-DNA Upon bifunctional activation of MC, poly(dG-dC)/Co(NH3)63+reverted to the B-form and bifunctional (cross-link) adducts were detected, identical again with those formed with B-DNA Poly(dG-m5dC), however, remained in the Z-form after the alkylation and only a monofunctional adduct could be detected. It was concluded that Z-DNA is subject to monofunctional alkylation by MC but cannot be cross-linked. The latter process occurs only when the Z-DNAis labile enough [as is in the case of poly(dG-dC)] to have some B-form in equilibrium at the site of the first formed monolinked adduct; the cross-linking then occurs at such local B-sites, pulling the overall B⇌Z equilibrium irreversibly to the left. These results are in accord with the predictions from the above modeling. The irreversible “lock” by the MC cross-link on B-DNA may be exploited for probing Z-DNA intermediacy in various DNA functions.
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基于 Z-DNA 亲和层析鉴定人类肿瘤细胞系的同源配对和链交换活性。
DOI：
10.1073/pnas.85.1.36
发表时间：
1988
影响因子：
11.1
作者：
Fishel,RA;Detmer,K;Rich,A
通讯作者：
Rich,A
黄曲霉毒素 B1 抑制盐诱导的 B-DNA 向 Z-DNA 的转化。
影响因子：
56.9
作者：
A. Nordheim;W. Hao;G. Wogan;A. Rich
通讯作者：
A. Rich
乙锭与左手 (Z) DNA 结合会在嵌入位点产生右手 DNA 区域。
影响因子：
2.9
作者：
Walker,GT;Stone,MP;Krugh,TR
通讯作者：
Krugh,TR
DOI：
--
发表时间：
1989
期刊：
影响因子：
--
作者：
A. Walter
通讯作者：
A. Walter
用反式-7,8-二羟基-9,10-环氧-7,8,9,10的反非对映异构体共价修饰后B和Z形式的双链聚d[(G-C)(G-C)]的光谱研究
影响因子：
4.7
作者：
I. Zegar;P. Lycksell;A. Gräslund;M. Eriksson;B. Nordén;B. Jernström
通讯作者：
B. Jernström