Molecular detection and genetic diversity of bovine Babesia spp., Theileria orientalis, and Anaplasma marginale in beef cattle in Thailand
Molecular detection and genetic diversity of bovine Babesia spp., Theileria orientalis, and Anaplasma marginale in beef cattle in Thailand
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DOI：
10.1007/s00436-016-5345-2
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发表时间：
2017-02-01
影响因子：
2
通讯作者：
Xuan, Xuenan
中科院分区：
文献类型：
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作者：
Jirapattharasate, Charoonluk;Moumouni, Paul Franck Adjou;Xuan, Xuenan
巴贝虫属，东方泰勒虫和边缘无浆体是重要的蜱传病原体，影响热带和亚热带地区牛的健康和生产力。在本研究中，我们采用PCR技术检测了牛巴伯虫、二联巴伯虫和T。orientalis和A.来自北部、东北部和西部地区的608头肉牛的边缘病。使用基于B的种特异性引物进行PCR。牛球状体蛋白2（BboSBP 2），B. bigemina rhoptry-associated protein 1a（BbiRAP-1a），T. orientalis major piroplasma surface protein（ToMPSP）和A.边缘主要表面蛋白4（AmMSP 4）基因。为了确定上述寄生虫的遗传多样性，扩增B。bovis和B.二联体ITS 1 - 5.8SrRNA基因-ITS 2区（B. bovis ITS，B. bigemina ITS）、ToMPSP和AmMSP 4基因进行系统发育分析。PCR结果显示B. bovis，牛湾（B. bigemina，T. orientalis和A.西部地区边缘型分别为11.1%、12.5%、7.8%和39.1%。在17.9%的采样动物中观察到两种或三种寄生虫的合并感染。研究表明，A.西部地区边缘型高于北部和东北部地区（7%）。序列分析表明BboSBP 2基因比B基因保守。BbiRAP-1a的保守性高于B。比吉米纳群岛在系统发育分析中，T. orientalis MPSP序列分为3、5和7型。A.边缘型MSP 4基因序列与国外分离株具有较高的同源性和相似性，并与国外分离株聚在一起。这项研究提供了关于肉牛蜱传病原体的流行和遗传多样性的信息，并强调了在泰国控制这些病原体的有效策略的必要性。
Babesia spp., Theileria orientalis, and Anaplasma marginale are significant tick-borne pathogens that affect the health and productivity of cattle in tropical and subtropical areas. In this study, we used PCR to detect the presence of Babesia bovis, Babesia bigemina, and T. orientalis in 279 beef cattle from Western Thailand and A. marginale in 608 beef cattle from the north, northeastern, and western regions. The PCRs were performed using species-specific primers based on the B. bovis spherical body protein 2 (BboSBP2), B. bigemina rhoptry-associated protein 1a (BbiRAP-1a), T. orientalis major piroplasm surface protein (ToMPSP), and A. marginale major surface protein 4 (AmMSP4) genes. To determine the genetic diversity of the above parasites, amplicons of B. bovis and B. bigemina ITS1-5.8s rRNA gene-ITS2 regions (B. bovis ITS, B. bigemina ITS), ToMPSP, and AmMSP4 genes were sequenced for phylogenetic analysis. PCR results revealed that the prevalence of B. bovis, B. bigemina, T. orientalis, and A. marginale in the Western region was 11.1, 12.5, 7.8, and 39.1 %, respectively. Coinfections of two or three parasites were observed in 17.9 % of the animals sampled. The study revealed that the prevalence of A. marginale in the western region was higher than in the north and northeastern regions (7 %). Sequence analysis showed the BboSBP2 gene to be more conserved than B. bovis ITS in the different isolates and, similarly, the BbiRAP-1a was more conserved than B. bigemina ITS. In the phylogenetic analysis, T. orientalis MPSP sequences were classified into types 3, 5, and 7 as previously reported. A. marginale MSP4 gene sequences shared high identity and similarity with each other and clustered with isolates from other countries. This study provides information on the prevalence and genetic diversity of tick-borne pathogens in beef cattle and highlights the need for effective strategies to control these pathogens in Thailand.