Identification of chicken embryo kinase 5, a developmentally regulated receptor-type tyrosine kinase of the Eph family.
Identification of chicken embryo kinase 5, a developmentally regulated receptor-type tyrosine kinase of the Eph family.
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鸡胚激酶 5 的鉴定，一种 Eph 家族发育调节受体型酪氨酸激酶。
DOI：
10.1091/mbc.2.7.523
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Pasquale,EB
中科院分区：
文献类型：
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作者：
Pasquale,EB
鸡胚激酶5（Chicken embryonic kinase 5，Cek 5）是通过用抗磷酸酪氨酸抗体筛选鸡胚cDNA表达文库而鉴定的Eph家族的跨膜酪氨酸激酶。Cek 5的胞外区含有富含半胱氨酸的N-末端亚结构域和C-末端亚结构域，其大多缺乏半胱氨酸并且包含两个类似于纤连蛋白III型重复的重复。抗Cek 5多克隆抗体的免疫印迹实验表明，Cek 5是一种膜相关的120 kDa的蛋白质，含有分子内（但不是分子间）二硫键。Cek 5已经在2天大的鸡胚中表达，并且在发育后期也以更高的水平表达。在10天大的鸡胚中，Cek 5在几乎所有检查的组织中以实质水平表达，而在成年鸡中，它主要在脑中表达。Cek 5在大脑中的表达在胚胎发育过程中逐渐减少，而在大腿的骨骼肌中，在终末肌肉分化时检测到Cek 5表达的急剧下降。其在整个发育过程中的广泛组织分布及其在成人脑中的持续表达表明Cek 5是信号转导途径的重要组分，可能与广泛分布的重要配体相互作用，这是未知的。
Chicken embryo kinase 5 (Cek5) is a transmembrane tyrosine kinase of the Eph family that was identified by screening a 10-d chicken embryo cDNA expression library with anti-phosphotyrosine antibodies. The extracellular region of Cek5 contains a cysteine rich N-terminal subdomain and a C-terminal subdomain mostly devoid of cysteines and comprising two repeats similar to fibronectin type III repeats. Immunoblotting experiments with anti-Cek5 polyclonal antibodies indicated that Cek5 is a membrane-associated 120-kDa protein containing intramolecular (but not intermolecular) disulfide bonds. Cek5 is already expressed in 2-d-old chicken embryos and is also expressed, at higher levels, later in development. In 10-d-old chicken embryos, Cek5 is expressed at substantial levels in nearly all the tissues examined, whereas in adult it is expressed predominantly in the brain. The expression of Cek5 in the brain gradually diminishes during embryonic development, whereas in the skeletal muscle of the thigh a sharp decrease in Cek5 expression was detected at the time of terminal muscle differentiation. Its wide tissue distribution throughout development and its sustained expression in adult brain suggest that Cek5 is an important component of signal transduction pathways, likely to interact with a widely distributed and important ligand, which is as yet unknown.