SEQUENCE DIVERSITY IN S1 GENES AND S1 TRANSLATION PRODUCTS OF 11 SEROTYPE-3 REOVIRUS STRAINS
SEQUENCE DIVERSITY IN S1 GENES AND S1 TRANSLATION PRODUCTS OF 11 SEROTYPE-3 REOVIRUS STRAINS
复制标题
DOI：
10.1128/jvi.64.10.4842-4850.1990
复制
发表时间：
1990-10-01
影响因子：
5.4
通讯作者：
FIELDS, BN
中科院分区：
文献类型：
--
作者：
DERMODY, TS;NIBERT, ML;FIELDS, BN
测定了10个3型（T3）呼肠孤病毒株的S1基因核苷酸序列，并与T3原型Dearing株进行比较，以研究单一呼肠孤病毒血清型的毒株中的序列多样性，并更多地了解两种S1翻译产物σ 1和r1 s的结构-功能关系。从编码σ 1的S1核苷酸序列的变异构建的系统发生树的分析表明，基于宿主物种、地理位置或分离数据，在这些菌株中不存在S1基因相关性的模式。这表明呼肠孤病毒株在宿主物种之间传播迅速，并且具有显著不同S1序列的T3株同时传播。T3菌株的推导的σ 1氨基酸序列的比较值得注意的是鉴定了与σ 1的所提出的结构域组织相关的序列的保守区和可变区（M.L. T.S.尼伯特德莫迪和B。N. Fields，J. Virol. 64：2976-2989，1990）。被认为对σ 1结构重要的非极性残基的重复模式在所有检测的菌株中是保守的。推导的菌株的σ 1 s氨基酸序列比σ 1序列更不均一;然而，σ 1 s氨基末端附近的一簇碱性残基是保守的。该分析使我们能够研究T3呼肠孤病毒株的分子流行病学并鉴定S1翻译产物σ 1和σ 1 s中的保守和可变序列基序。
The S1 gene nucleotide sequences of 10 type 3 (T3) reovirus strains were determined and compared with the T3 prototype Dearing strain in order to study sequence diversity in strains of a single reovirus serotype and to learn more about structure-function relationships of the two S1 translation products, .sigma.1 and r1s. Analysis of phylogenetic trees constructed from variation in the .sigma.1-encoding S1 nucleotide sequences indicated that there is no pattern of S1 gene relatedness in these strains based on host species, geographic site, or data of isolation. This suggests that reovirus strains are transmitted rapidly between host species and that T3 strains with markedly different S1 sequences circulate simultaneously. Comparison of the deduced .sigma.1 amino acid sequences of the T3 strains was notable for the identification of conserved and variable regions of sequence that correlate with the proposed domain organization of .sigma.1 (M.L. Nibert, T.S. Dermody, and B. N. Fields, J. Virol. 64:2976-2989, 1990). Repeat patterns of apolar residues thought to be important for .sigma.1 structure were conserved in all strains examined. The deduced .sigma.1s amino acid sequences of the strains were more heterogeneous than the .sigma.1 sequences; however, a cluster of basic residues near the amino terminus of .sigma.1s was conserved. This analysis has allowed us to investigate molecular epidemiology of T3 reovirus strains and to identify conserved and variable sequence motifs in the S1 translation products, .sigma.1 and .sigma.1s.