Effective cell-seeding technique using magnetite nanoparticles and magnetic force onto decellularized blood vessels for vascular tissue engineering
Effective cell-seeding technique using magnetite nanoparticles and magnetic force onto decellularized blood vessels for vascular tissue engineering
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DOI：
10.1263/jbb.103.472
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发表时间：
2007-05-01
影响因子：
2.8
通讯作者：
Honda, Hiroyuki
中科院分区：
文献类型：
--
作者：
Shimizu, Kazunori;Ito, Akira;Honda, Hiroyuki
近年来，血管组织工程越来越受到人们的关注。虽然细胞接种到管状支架上是构建三维血管移植物的第一步，但移植物的管状几何形状阻碍了细胞有效地递送到支架上。为了克服这些限制，我们在这里提出了一种使用磁力和磁铁矿纳米颗粒的新型细胞接种技术，称为Mag-seeding。NIH/3 T3成纤维细胞（3 T3）磁性标记使用我们原来的磁铁矿阳离子脂质体（MCL），它具有正表面电荷，以提高吸附到细胞表面。在这项研究中，猪脱细胞颈总动脉（dCCA）被用作最有前途的支架之一，因为dCCA由构成细胞外基质的结构和功能蛋白的混合物组成。当将圆柱形磁体插入dCCA的内腔中并将dCCA浸入磁性标记的3 T3的悬浮液中时，几乎所有的3 T3都附着在dCCA上，而在不使用磁体的情况下实现了低的细胞接种效率。当每个细胞的磁铁矿摄取率增加，细胞接种效率的磁接种增强。此外，为了构建用于人的血管移植物，通过Mag-seeding成功构建了用两种人细胞（平滑肌细胞和真皮成纤维细胞）重新接种的猪dCCA。这些结果表明，磁接种可以用于血管组织工程。
Increasing attention has been given to vascular tissue engineering in recent years. Although cell seeding onto tubular scaffolds is the first step for constructing three-dimensional vascular grafts, the tubular geometry of the grafts hinders the efficient delivery of cells onto the scaffold. To overcome these limitations, we present here a novel cell-seeding technique using magnetic force and magnetite nanoparticles, termed Mag-seeding. NIH/3T3 fibroblasts (3T3s) were labeled magnetically using our original magnetite cationic liposomes (MCLs), which have a positive surface charge, to improve adsorption onto cell surface. In this study, porcine decellularized common carotid artery (dCCA) was used as one of the most promising scaffolds, because dCCA consists of a mixture of structural and functional proteins that constitute the extracellular matrix. When a cylindrical magnet was inserted into the lumen of dCCA and the dCCA was immersed into a suspension of magnetically labeled 3T3s, almost all the 3T3s attached onto the dCCA, whereas a low cell-seeding efficiency was achieved without using a magnet. When the magnetite uptake rate per cell increased, cell-seeding efficiency by Mag-seeding was enhanced. Furthermore, to construct a vascular graft for humans, the porcine dCCA, which was reseeded with two human cells (smooth muscle cells and dermal fibroblasts), was successfully constructed by Mag-seeding. These results indicate that Mag-seeding can be used for vascular tissue engineering.