Expression of the epithelial marker E-cadherin by thyroid C cells and their precursors during murine development
Expression of the epithelial marker E-cadherin by thyroid C cells and their precursors during murine development
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DOI：
10.1369/jhc.7a7179.2007
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发表时间：
2007-10-01
影响因子：
3.2
通讯作者：
Sucov, Henry M.
中科院分区：
文献类型：
--
作者：
Kameda, Yoko;Nishimaki, Toshiyuki;Sucov, Henry M.
对鸡-鹌鹑嵌合体的研究表明，鸟类的超鳃裂C细胞起源于神经嵴。因此，在没有太多证据支持的情况下，人们假设哺乳动物的甲状腺C细胞也起源于神经嵴。为了验证这一观点，我们使用了Connexin43-lacZ和Wnt1-Cre/R26R转基因小鼠，因为它们的神经嵴细胞可以被标记。我们还检测了一些识别迁移或迁移后神经嵴细胞的标记物的免疫组织化学表达，即TuJ1、神经丝160、巢蛋白、P75NTR和Sox10。此外，我们检测了上皮细胞标志物E-cadherin的表达。在胚胎日(E)10.5时，神经嵴细胞密集分布于咽弓，但未分布于咽袋，包括第四个咽袋。在E11.5时，鳃裂雏形由第四个囊形成，位于第四个弓动脉附近。在13岁时，这个器官与甲状腺叶接触，在13岁时，它与甲状腺叶融合。然而，在这些发育阶段，终鳃体并没有被神经嵴来源的细胞定植。相反，所有的鳃末细胞，以及第四咽袋上皮，对e -钙粘蛋白有强烈的免疫反应。此外，新生小鼠甲状腺的共聚焦显微镜显示降钙素和e -钙粘蛋白在C细胞中共定位。然而，这些细胞在Wnt-Cre/R26R小鼠中没有被标记。这些结果表明，小鼠甲状腺C细胞来源于第四咽袋的内胚层上皮细胞，而不是来源于神经嵴细胞。
Studies of chick-quail chimeras have reported that avian ultimobranchial C cells originate from the neural crest. It has consequently been assumed, without much supporting evidence, that mammalian thyroid C cells also originate from the neural crest. To test this notion, we employed both Connexin43-lacZ and Wnt1-Cre/R26R transgenic mice, because their neural crest cells can be marked. We also examined the immunohistochemical expression of a number of markers that identify migratory or postmigratory neural crest cells, namely, TuJ1, neurofilament 160, nestin, P75NTR, and Sox10. Moreover, we examined the expression of E-cadherin, an epithelial cell marker. At embryonic day (E)10.5, the neural crest cells densely populated the pharyngeal arches but were not distributed in the pharyngeal pouches, including the fourth pouch. At E11.5, the ultimobranchial rudiment formed from the fourth pouch and was located close to the fourth arch artery. At E13.0, this organ came into contact with the thyroid lobe, and at E13.5, it fused with this lobe. However, the ultimobranchial body was not colonized by neural crest-derived cells at any of these developmental stages. Instead, all ultimobranchial cells, as well as the epithelium of the fourth pharyngeal pouch, were intensely immunoreactive for E-cadherin. Furthermore, confocal microscopy of newborn mouse thyroid glands revealed colocalization of calcitonin and E-cadherin in the C cells. The cells, however, were not marked in the Wnt-Cre/R26R mice. These results indicated that murine thyroid C cells are derived from the endodermal epithelial cells of the fourth pharyngeal pouch and do not originate from neural crest cells.