Characterization of mouse helper and suppressor T cell subsets separated by lectins.
Characterization of mouse helper and suppressor T cell subsets separated by lectins.
复制标题
通过凝集素分离的小鼠辅助性 T 细胞和抑制性 T 细胞亚群的表征。
作者：
T. Nakano;Y. Imai;M. Naiki;T. Osawa
通过分离与花生凝集素（PNA）凝集的细胞来分级分离伴刀豆球蛋白A（Con A）活化的细胞，花生凝集素（PNA）优先结合糖序列β-D-Gal-（1导致3）-D-GalNAc。发现PNA凝集细胞对绵羊红细胞的初级抗体反应产生显著的抑制作用，而PNA非凝集细胞不影响抗体反应。PNA凝集抑制细胞对抗Thy 1.2和补体（C）、抗I-J抗血清和补体、抗Ly 2.2抗血清和补体均敏感。这些结果表明，PNA凝集抑制细胞，从刀豆蛋白A激活的细胞分离，T细胞，这些抑制T细胞具有丰富的β-半乳糖残基暴露在细胞表面上，而不是由唾液酸残基掩蔽。此外，发现抗原特异性抑制细胞也可通过使用与PNA的凝集性分离。PNA-凝集抑制T细胞活性被消除的Con A-激活的PNA-凝集细胞与抗-去唾液酸GM 1和补体，抗-Forssman抗血清和补体，和抗-globoside抗血清和补体的治疗，但不通过治疗与抗-GM 1抗血清和补体。相反，由透镜培养物凝集素诱导并由多汁鲎凝集素分离的辅助性T细胞活性通过用抗GM 1和补体处理细胞而消除，但不通过用其它抗糖脂抗血清和补体处理而消除。
Concanavalin A (Con A)-activated cells were fractionated by separating cells that agglutinate with peanut agglutinin (PNA) which binds preferentially to the sugar sequence beta-D-Gal-(1 leads to 3)-D-GalNAc. PNA-agglutinable cells were found to exert a significant suppressor effect on the primary antibody response to sheep red blood cells, whereas PNA-nonagglutinable cells did not affect the antibody response. It was also found that PNA-agglutinable suppressor cells were sensitive to treatment with anti-Thy 1.2 and complement (C), anti-I-J antiserum and complement, and anti-Ly 2.2 antiserum and complement. These results suggest that the PNA-agglutinable suppressor cells, separated from Con A-activated cells, were T cells and that these suppressor T cells possess abundant beta-galactosyl residues exposed on the cell surface, not masked by sialyl residues. Furthermore, it was found that antigen-specific suppressor cells were also separable by use of the agglutinability with PNA. The PNA-agglutinable suppressor T cell activity was eliminated by treatment of the Con A-activated PNA-agglutinable cells with anti-asialo GM1 and complement, anti-Forssman antiserum and complement, and anti-globoside antiserum and complement, but not by treatment with anti-GM1 antiserum and complement. Conversely, the helper T cell activity that was induced by Lens culinalis agglutinin and separated by Limulus polyphemus agglutinin, was eliminated by treatment of the cells with anti-GM1 and complement, but not by the treatment with the other anti-glycolipid antisera and complement.