Diversity in transcriptional start site selection and alternative splicing affects the 5'-UTR of mouse striated muscle myosin transcripts.
Diversity in transcriptional start site selection and alternative splicing affects the 5'-UTR of mouse striated muscle myosin transcripts.
复制标题
转录起始位点选择和选择性剪接的多样性影响小鼠横纹肌肌球蛋白转录物的 5-UTR。
DOI：
10.1007/s10974-006-9071-8
复制
发表时间：
2006
影响因子：
2.7
通讯作者：
Krauter,KennethS
中科院分区：
文献类型：
--
作者：
Dennehey,BrianaK;Leinwand,LeslieA;Krauter,KennethS
我们分析了近2000个肌球蛋白重链基因（Myh）克隆，代表了小鼠横纹肌Myh基因表达的8个中的7个的30多个不同转录本。我们还报道了小鼠发育myhgenes的转录起始位点（TSS）。这些数据揭示了以前未知的tss多样性和这些转录本中的5 '端选择性剪接。cardiacmyh6基因有两个主要的tss。主要下游位点的使用导致第二外显子的选择性剪接。其他每个myhgenes都有一个主要的TSS和一个或多个次要的TSS，其中一些与选择性剪接有关。次要转录本与多体相关，它们的时空表达在很大程度上反映了野生型、Myh1null、Myh4null、损伤和未损伤肌肉中的主要转录本，除了在心脏中检测到的myh7的一种形式在膈肌中未检测到，并且两种主要myh6转录本的比例在某些情况下有所不同。这些发现表明，TSS的选择性使用和5 ' -UTR的选择性剪接是小鼠myhgene表达的一个普遍特征，并且myhgene的调控比以前认为的要复杂得多。
We have analyzed nearly 2,000 myosin heavy chain gene (Myh) clones representing over 30 different transcripts from seven of eight striated muscleMyhgenes expressed in mouse. We also report the transcriptional start sites (TSS) for the mouse developmentalMyhgenes. The data reveal a previously unknown diversity of TSSs and 5′-end alternative splicing in these transcripts. The cardiacMyh6gene had two major TSSs. Use of the major downstream site led to an alternatively spliced second exon. Each of the otherMyhgenes had one major TATA-directed TSS and one or more minor alternative TSSs, some associated with alternative splicing. The minor transcripts were associated with polysomes and their spatial-temporal expression largely mirrored that of the major transcripts in wild-type,Myh1null,Myh4null, injured, and uninjured muscle, except that one form ofMyh7, detected in heart, was not detected in diaphragm, and the ratio of the two majorMyh6transcripts varied in some circumstances. These findings indicate that alternative TSS usage and alternative splicing in the 5′-UTR are a general feature of murineMyhgene expression and thatMyhgene regulation is more complex than previously appreciated.