Higher production of IL-8 in visceral vs. subcutaneous adipose tissue. Implication of nonadipose cells in adipose tissue
Higher production of IL-8 in visceral vs. subcutaneous adipose tissue. Implication of nonadipose cells in adipose tissue
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DOI：
10.1152/ajpendo.00269.2003
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发表时间：
2004-01-01
影响因子：
5.1
通讯作者：
Richelsen, B
中科院分区：
文献类型：
--
作者：
Bruun, JM;Lihn, AS;Richelsen, B
IL-8 从人体脂肪组织中释放。与瘦人相比，肥胖者的循环 IL-8 增加，并且与胰岛素抵抗、动脉粥样硬化的发展和心血管疾病相关。我们研究了 1) 体外皮下 (SAT) 和内脏 (VAT) 脂肪组织配对样品中 IL-8 的产生和释放，以及 2) 整个脂肪组织、分离的脂肪细胞和脂肪组织的非脂肪细胞中 IL-8 的产生。 VAT 中的 IL-8 释放量是 SAT 中的四倍（P < 0.05），VAT 中的 IL-8 mRNA 是 SAT 中的两倍（P < 0.01）。地塞米松 (50 nM) 使 IL-8 产量减少 50% (P < 0.05)，IL-1β (2 μg/l) 使 IL-8 产量增加达 15 倍 (P < 0.001)。整个 SAT 外植体释放的 IL-8 与体重指数相关（BMI；r = 0.78；P < 0.001），非脂肪细胞释放的 IL-8 也与体重指数相关（r = 0.79；P < 0.001）。然而，分离脂肪细胞部分的 IL-8 释放量与 BMI 之间没有相关性 (r = 0.01)。总之，我们证明与 SAT 相比，VAT 中 IL-8 的释放量有所增加。此外，我们的数据表明，在肥胖受试者中观察到的IL-8循环水平升高主要是由于脂肪组织的非脂肪细胞释放IL-8。人脂肪组织中高水平的IL-8释放以及肥胖受试者中该组织的积累可能是在肥胖中观察到的循环IL-8增加的部分原因。
IL-8 is released from human adipose tissue. Circulating IL-8 is increased in obese compared with lean subjects and is associated with measures of insulin resistance, development of atherosclerosis, and cardiovascular disease. We studied 1) the production and release of IL-8 in vitro from paired samples of subcutaneous ( SAT) and visceral ( VAT) adipose tissue and 2) the production of IL-8 from whole adipose tissue, isolated adipocytes, and nonfat cells of adipose tissue. IL-8 release from VAT was fourfold higher than from SAT ( P < 0.05), and IL-8 mRNA was twofold higher in VAT compared with SAT (P < 0.01). Dexamethasone ( 50 nM) attenuated IL-8 production by 50% (P < 0.05), and IL-1β (2 μg/l) increased IL-8 production up to 15-fold ( P < 0.001). IL- 8 release from whole SAT explants correlated with body mass index (BMI; r = 0.78; P < 0.001), as did IL-8 release from nonfat cells (r = 0.79; P < 0.001). However, no correlation was found between IL-8 release from the fraction of isolated adipocytes and BMI ( r = 0.01). In conclusion, we demonstrated an increased release of IL- 8 from VAT compared with SAT. Furthermore, our data suggest that the observed elevation in circulating levels of IL- 8 in obese subjects is due primarily to the release of IL- 8 from nonfat cells from adipose tissue. The high levels of IL- 8 release from human adipose tissue and accumulation of this tissue in obese subjects may account for some of the increase in circulating IL- 8 observed in obesity.