Evaluation of miRNA-21 and miRNA Let-7 as Prognostic Markers in Patients With Breast Cancer
Evaluation of miRNA-21 and miRNA Let-7 as Prognostic Markers in Patients With Breast Cancer
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DOI：
10.1016/j.clbc.2017.11.022
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发表时间：
2018-08-01
影响因子：
3.1
通讯作者：
Ibrahim, Mona H.
中科院分区：
文献类型：
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作者：
Elghoroury, Eman A.;ElDine, Hesham G.;Ibrahim, Mona H.
乳腺癌是一个具有挑战性的医疗保健问题。我们检测了3组患者外周血中miRNA-21和miRNA let-7的水平。MiRNA-21在乳腺癌中高表达，而miRNA let-7在乳腺癌中表达显着降低，并与乳腺癌转移的风险有关。MiRNA let-7作为乳腺癌的预后标志物而出现。背景：乳腺癌仍然是女性健康的最大威胁之一。目前缺乏具有理想的敏感性和特异性的肿瘤标志物是未来乳腺癌治疗的主要障碍。许多研究旨在揭示循环miRNAs在乳腺癌中的诊断和预后潜力。在这项研究中，我们试图评估循环miRNA-21和let-7作为乳腺癌预后生物标志物的可行性和临床应用价值。方法：采用实时荧光定量聚合酶链式反应技术。检测3组125名受试者血清中miRNA-21和let-7的表达水平。用折叠变化分析法检测各组中miRNA-21和let-7的表达。结果：乳腺癌患者miRNA-21的表达显著高于对照组和其他乳腺良性病变患者(P<；.001)。乳腺癌患者血清miRNA-21的平均表达水平为3.27+/-2.10倍。乳腺癌组织中miRNA let-7的表达(2.45+/-2.20倍)显著低于对照组(5.27+/-3.30倍)和乳腺良性病变组(6.22+/-4.90倍；P<；.001)。在乳腺癌患者中，miRNA let-7的表达水平与转移的发展呈负相关(P<；.001)。结论：我们的研究建立了乳腺癌患者miRNA let-7水平改变与转移风险之间的联系，暗示miRNA let-7在疾病进展和预后中发挥作用。(C)2017 Elsevier Inc.保留所有权利。
Cancer breast is a challenging health care problem. We studied the circulating levels of miRNA-21 and miRNA let-7 in 3 different groups. miRNA-21 was highly expressed in patients with breast cancer whereas miRNA let-7 expression was markedly reduced and correlated with risk development of metastases in breast cancer cases. miRNA let-7 arises as a prognostic marker for cancer breast.Background: Breast cancer remains one of the top threats to women's health. The current lack of tumor markers with desirable sensitivity and specificity is a major obstacle toward the future management of breast cancer. Many studies are directed to reveal the diagnostic and prognostic potentials of circulating miRNAs in breast cancer. In this study, we attempt to evaluate the feasibility and clinical utility of circulating miRNA-21 and let-7 as prognostic biomarkers for breast cancer. Methods: Real-time quantitative polymerase chain reaction technique was used. Levels of miRNA-21 and let-7 expression were determined in sera from 125 participants representing 3 different groups. With fold-change analysis, the expression of miRNA-21 and let-7 in the decided groups were assessed. Results: Patients with breast cancer showed significantly higher expression of miRNA-21 compared with controls and other participants with benign breast lesions (P < .001). The mean expression levels of serum miRNA-21 was 3.27 +/- 2.10-fold in patients with breast cancer. The expression of miRNA let-7 was significantly decreased in patients with breast cancer (2.45 +/- 2.20 fold) than the control group and the benign breast lesions group (5.27 +/- 3.30-fold and 6.22 +/- 4.90-fold, respectively; P < .001). Levels of miRNA let-7 expression negatively correlated with development of metastases in patients with breast cancer (P < .001). Conclusions: Our study establishes the association between altered levels of miRNA let-7 and metastases risk in patients with breast cancer, implying a role of miRNA let-7 in disease progression and prognosis. (C) 2017 Elsevier Inc. All rights reserved.