A Biomimetic Collagen-Apatite Scaffold with a Multi-Level Lamellar Structure for Bone Tissue Engineering.
A Biomimetic Collagen-Apatite Scaffold with a Multi-Level Lamellar Structure for Bone Tissue Engineering.
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DOI：
10.1039/c3tb21595d
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发表时间：
2014-04-14
期刊：
影响因子：
--
通讯作者：
Wei M
中科院分区：
文献类型：
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作者：
Xia Z;Villa MM;Wei M
胶原-磷灰石(COL-AP)支架已被广泛应用于骨组织工程。我们制备了一种具有独特的层状结构的COL-AP支架，该结构由共排列的微孔和宏孔组成。这种支架的基本构件是类骨矿化的胶原纤维，它是在含有胶原的改性模拟体液(m-SBF)中通过仿生自组装过程形成的。这种仿生方法通过用磷灰石纳米颗粒增强胶原水凝胶，保持了胶原的结构完整性和巨大的抗张强度。通过控制冻结速度和冻结方向，可以形成大小为63.8~344μm的定向排列的大孔。COL-AP片层厚度可根据自压缩时间在3.6~23μm范围内调节。此外，与具有各向同性等轴孔结构的相同成分的支架相比，多层片状结构导致沿取向方向的杨氏模量和压缩模量分别增加了12倍和2倍。此外，这种新型的板层支架支持MC3T3-E1成骨细胞的附着和铺展。因此，由于这种新型支架的仿生组成、结构可调、机械强度提高以及良好的生物相容性，它在骨组织工程中具有巨大的应用潜力。
Collagen-apatite (Col-Ap) scaffolds have been widely employed for bone tissue engineering. We fabricated a Col-Ap scaffold with a unique multi-level lamellar structure consisting of co-aligned micro and macro pores. The basic building blocks of this scaffold are bone-like mineralized collagen fibers developed via a biomimetic self-assembly process in a collagen-containing modified simulated body fluid (m-SBF). This biomimetic method preserves the structural integrity and great tensile strength of collagen by reinforcing the collagen hydrogel with apatite nano-particles. Unidirectional aligned macro pores with a size of 63.8 to 344 μm are created by controlling the freezing rate and direction. The thickness of Col-Ap lamellae can be adjusted in the range 3.6 to 23 μm depending on the self-compression time. Furthermore, the multi-level lamellar structure has led to a twelve-fold increase in Young's modulus and a two-fold increase in the compression modulus along the aligned direction compared to a scaffold of the same composition with an isotropic equiaxed pore structure. Moreover, this novel lamellar scaffold supports the attachment and spreading of MC3T3-E1osteoblasts. Therefore, owing to the biomimetic composition, tunable structure, improved mechanical strength, and good biocompatibility of this novel scaffold, it has great potential to be used in bone tissue engineering applications.