Simple and longstanding adipose tissue engineering in rabbits
Simple and longstanding adipose tissue engineering in rabbits
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DOI：
10.1007/s10047-012-0670-4
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发表时间：
2013-03-01
影响因子：
1.3
通讯作者：
Toi, Masakazu
中科院分区：
文献类型：
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作者：
Tsuji, Wakako;Inamoto, Takashi;Toi, Masakazu
脂肪组织工程乳房再造可用于接受乳房手术的患者。我们先前已经证实了在小鼠中植入具有成纤维细胞生长因子2（FGF 2）的受控释放的I型胶原海绵和人脂肪组织来源的干细胞的脂肪形成。然而，为了使用这种方法来治疗乳腺癌患者，需要大量的脂肪组织，而FGF 2并不容易获得。因此，我们的目标是在没有FGF 2的情况下长时间再生大量脂肪组织。在全身麻醉下，将聚丙烯网制成的笼植入兔的双侧脂肪垫中。每个融合器的半径为10 mm，高度为10 mm。将切碎的I型胶原海绵作为支架注射到笼中。用超声检查融合器中的再生组织，并在植入后3、6和12个月收获融合器。超声检查显示椎间融合器内有一个逐渐增加的均匀高回声区。用苏木精和伊红染色评估标本的组织学。在植入后6个月和12个月，再生脂肪组织面积百分比分别为76.2 +/- A 13.0和92.8 +/- A 6.6%。我们的研究结果显示，在间隙内仅植入I型胶原海绵后12个月，新生脂肪形成。超声检查是评估融合器内组织生长的一种无创且有用的方法。这种简单的方法可能是一个有前途的临床模式在乳房再造。
Adipose tissue engineering for breast reconstruction can be performed for patients who have undergone breast surgery. We have previously confirmed adipogenesis in mice implanted with type I collagen sponge with controlled release of fibroblast growth factor 2 (FGF2) and human adipose tissue-derived stem cells. However, in order to use this approach to treat breast cancer patients, a large amount of adipose tissue is needed, and FGF2 is not readily available. Thus, we aimed to regenerate large amounts of adipose tissue without FGF2 for a long period. Under general anesthesia, cages made of polypropylene mesh were implanted into the rabbits' bilateral fat pads. Each cage was 10 mm in radius and 10 mm in height. Minced type I collagen sponge was injected as a scaffold into the cage. Regenerated tissue in the cage was examined with ultrasonography, and the cages were harvested 3, 6, and 12 months after the implantation. Ultrasonography revealed a gradually increasing homogeneous high-echo area in the cage. Histology of the specimen was assessed with hematoxylin and eosin staining. The percentages of regenerated adipose tissue area were 76.2 +/- A 13.0 and 92.8 +/- A 6.6 % at 6 and 12 months after the implantation, respectively. Our results showed de novo adipogenesis 12 months after the implantation of only type I collagen sponge inside the space. Ultrasonography is a noninvasive and useful method of assessing the growth of the tissue inside the cage. This simple method could be a promising clinical modality in breast reconstruction.