The non-viability of uniparental mouse conceptuses correlates with the loss of the products of imprinted genes
The non-viability of uniparental mouse conceptuses correlates with the loss of the products of imprinted genes
复制标题
DOI：
10.1016/0925-4773(94)90037-x
复制
发表时间：
1994-04
影响因子：
2.6
通讯作者：
C. Walsh;A. Glaser;R. Fundele;A. Ferguson-Smith;S. Barton;M. Surani;R. Ohlsson
中科院分区：
文献类型：
--
作者：
C. Walsh;A. Glaser;R. Fundele;A. Ferguson-Smith;S. Barton;M. Surani;R. Ohlsson
二倍体孤雌生殖或雄核发育小鼠孕体产生特征性和相反的突变表型，并且是不可存活的，这可能是由于来自母本和父本基因组的不同贡献。这很可能是由于在后代中只有一个父母的特定基因拷贝优先表达的结果。到目前为止，已知有四个这样的内源性印记基因：父亲的等位基因Igf2和Snrp，母亲的等位基因Igf2和H19是活跃的，而它们的相反的父母等位基因是不活跃的。在这里，我们表明，表达模式的theIgf2和Igf2r基因在雄激素和孤雌生殖的概念与父母的等位基因通常表达它们，这意味着印记可以保持在没有其他父母的基因组这些基因。这也表明这两种类型的单亲孕体都缺乏发育重要的基因产物。然而，我们确实发现，H19基因不仅在孤雌生殖的孕体中高度表达，而且在雄激素发生的胎盘中的巨大滋养层和次级巨细胞中也高度表达，尽管H19基因在正常小鼠的胚胎外组织中具有印记。我们讨论了这些意见方面的非可行性的单亲概念和互惠印迹模式的theIgf2和H19基因。
Diploid parthenogenetic or androgenetic mouse conceptuses produce characteristic and opposite mutant phenotypes and are non-viable, presumably due to different contributions from the maternal and paternal genomes. This is likely to be the result of the preferential expression of only one parent's copy of certain genes in the offspring. So far, four such endogenous imprinted genes are known: the paternal alleles ofIgf2andSnrpnand the maternal alleles ofIgf2randH19are active, while their opposite parental alleles are inactive. Here we demonstrate that the expression patterns of theIgf2andIgf2rgenes in androgenetic and parthenogenetic conceptuses correlate with which parental alleles normally express them, implying that the imprint can be maintained in the absence of the other parent's genome for these genes. This also indicates that both types of uniparental conceptuses are lacking developmentally important gene products. We did find, however, that theH19gene was highly expressed not only in the parthenogenetic conceptus, but also in giant trophoblasts and secondary giant cells in the androgenetic placenta, in spite of the imprinting of theH19gene in normal mouse extra embryonic tissues. We discuss these observations with respect to the non-viability of uniparental conceptuses and the reciprocal imprinting patterns of theIgf2andH19genes.