Cell culture models of the ocular barriers
Cell culture models of the ocular barriers
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DOI：
10.1016/j.ejpb.2005.01.009
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发表时间：
2005-07-01
影响因子：
4.9
通讯作者：
Urtti, A
中科院分区：
文献类型：
--
作者：
Hornof, M;Toropainen, E;Urtti, A
紧密屏障的存在调节眼睛前部和后部的眼组织环境，对于正常的视觉功能至关重要。制定策略来克服靶向眼部药物递送的这些障碍，例如对于视网膜来说，仍然是一个重大挑战。在过去的几年中，已经建立了许多眼屏障（角膜、结膜、血视网膜屏障）的细胞培养模型。它们被认为是研究药物转运到眼组织以及许多其他目的的有前景的工具，例如病理性眼部疾病的研究以及作为体内毒性测试替代的化合物毒理学筛选。这些体外模型的进一步开发将需要对细胞培养模型和体内眼屏障的屏障特性进行更详细的研究。本综述的目的是描述眼屏障细胞培养模型的发展现状，并讨论这些模型在药物研究中的适用性。 (c) 2005 Elsevier B.V. 保留所有权利。
The presence of tight barriers, which regulate the environment of ocular tissues in the anterior and posterior part of the eye, is essential for normal visual function. The development of strategies to overcome these barriers for the targeted ocular delivery of drugs, e.g. to the retina, remains a major challenge. During the last years numerous cell culture models of the ocular barriers (cornea, conjunctiva, blood-retinal barrier) have been established. They are considered to be promising tools for studying the drug transport into ocular tissues, and for numerous other purposes, such as the investigation of pathological ocular conditions, and the toxicological screening of compounds as alternative to in vivo toxicity tests. The further development of these in vitro models will require more detailed investigations of the barrier properties of both the cell culture models and the in vivo ocular barriers. It is the aim of this review to describe the current status in the development of cell culture models of the ocular barriers, and to discuss the applicability of these models in pharmaceutical research. (c) 2005 Elsevier B.V. All rights reserved.