The localisation of HSP70 and oxidative stress indices in heads of Spodoptera exigua larvae in a cadmium-exposed population
The localisation of HSP70 and oxidative stress indices in heads of Spodoptera exigua larvae in a cadmium-exposed population
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DOI：
10.1016/j.ecoenv.2011.10.024
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发表时间：
2012-04-01
影响因子：
6.8
通讯作者：
Swiergosz-Kowalewska, Renata
中科院分区：
文献类型：
--
作者：
Kafel, Alina;Nowak, Aleksandra;Swiergosz-Kowalewska, Renata
镉毒性的影响可能会有所不同的动物与不同的金属接触史。我们的研究的目的是探讨热休克蛋白70，蛋白质羰基水平，过氧化氢酶活性和总抗氧化能力的甜菜夜蛾（Hubner）幼虫的头部起源于经历土地44代镉处理和对照（那些没有暴露于镉）。结果表明，多代重金属处理的幼鱼头部热休克蛋白（HSPs）含量明显高于单代处理的幼鱼（对照组）。HSP定位在幼虫的大脑中的分析表明，这些变化可能是重要的保护神经功能的幼虫蘑菇体在多代金属暴露期间选择的动物。动物从一个世代的治疗，反过来，更高的总抗氧化能力比动物从多代治疗。无论如何，动物从一个和44代金属处理没有不同的金属积累在头部和整个幼虫体，过氧化氢酶活性或DNA损伤水平。所有这些测量值均高于对照幼虫，头部镉积累量远低于全身。(C)2011年由Elsevier Inc.出版
The effects of cadmium toxicity may vary between animals with different history of metal exposure. The aim of our study was to examine HSP70, protein carbonyl levels, catalase activity and total antioxidant capacity in the heads of Spodoptera exigua (Hubner) larvae originated from undergoing land 44-generational cadmium treatment and in control (those that were not exposed to cadmium). We also measured the cadmium concentration and DNA damage level in the larvae.We observed higher level of heat shock proteins (HSPs) in the heads of larvae derived from multi-generational metal treatment than in the heads of those from one-generational treatment (derived from the control rearing). Analysis of HSP localisation in the larval brain suggests that these changes could be important for protecting the neural function of larval mushroom bodies for animals selected during multigenerational metal exposure. Animals from one-generational treatment had, in turn, higher total antioxidant capacity than animals from multigenerational treatment. Anyway, animals from one- and 44-generational metal treatments did not differ in metal accumulation in the heads and the whole larval bodies, catalase activity or DNA damage level. All these measurements were higher than for control larvae and cadmium accumulation in the heads was much lower than in the whole bodies. (C) 2011 Published by Elsevier Inc.