The befores and afters of molecular replacement.
The befores and afters of molecular replacement.
复制标题
分子置换的贝福和之后。
DOI：
10.1107/s0907444907049736
复制
发表时间：
2008-01
期刊：
影响因子：
--
通讯作者：
Dodson, Eleanor
中科院分区：
文献类型：
--
作者：
Dodson, Eleanor
This review outlines questions to consider when attempting to solve crystal structures by molecular replacement. This review addresses the essential questions to consider when attempting to phase a new crystal structure using molecular replacement. Sequence matching can suggest whether there is a suitable three-dimensional model available, but it is also important to analyse the model in order to find its likely oligomeric state and to establish whether there are likely to be domain movements. Once a solution has been found it must be refined, which can be challenging for low-homology models. There is a detailed discussion of structures used as examples for CCP4 tutorials.
