In vitro inactivation of plasma alpha 1-proteinase inhibitor by epoxides and 1,2-dihaloethanes.
In vitro inactivation of plasma alpha 1-proteinase inhibitor by epoxides and 1,2-dihaloethanes.
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环氧化物和 1,2-二卤乙烷对血浆 α1-蛋白酶抑制剂的体外灭活。
DOI：
10.1007/bf01055521
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发表时间：
1988
影响因子：
4
通讯作者：
Barton,BK
中科院分区：
文献类型：
--
作者：
Ansari,GA;Gan,JC;Barton,BK
本文研究了三种环氧化合物和两种二卤乙烷对α_1-蛋白酶抑制剂及血浆本身的蛋白酶抑制活性的灭活作用。所有的三种环氧化物（环氧苯乙烯，环氧乙烷和环氧丙烷），单独，灭活弹性蛋白酶抑制剂或胰蛋白酶抑制活性的蛋白酶抑制剂，和整个血浆。氧化苯乙烯是纯化的α1-蛋白酶抑制剂或血浆的最有效灭活剂，其次是环氧乙烷和环氧丙烷，效力递减。二卤代乙烷、1，2-二氯乙烷和1，2-二溴乙烷也能使α1-蛋白酶抑制剂的蛋白酶抑制活性失活，前者比后者更有效。还观察到这些化学品对氨基的伴随修饰。
The inactivating effects of three epoxides and two dihaloethanes on the proteinase inhibitory activities of α1-proteinase inhibitor, as well as those of the plasma itself, were investigated. All of the three epoxides (styrene oxide, ethylene oxide and propylene oxide), individually, inactivated the elastase inhibitory or trypsin inhibitory activity of proteinase inhibitor, and that of whole plasma. Styrene oxide is the most effective inactivator of either purified α1-proteinase inhibitor or plasma, followed by ethylene oxide and propylene oxide in a decreasing order of potency. The dihaloethanes, 1,2-dichloroethane and 1,2-dibromoethane, also inactivated the proteinase inhibitory activities of α1-proteinase inhibitor with the former being somewhat more effective than the latter. Concomitant modification of the amino group was also observed with these chemicals.