Multifunctional Collagen and Hyaluronic Acid Multilayer Films on Live Mesenchymal Stem Cells
Multifunctional Collagen and Hyaluronic Acid Multilayer Films on Live Mesenchymal Stem Cells
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DOI：
10.1021/acsami.7b00365
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发表时间：
2017-04-12
影响因子：
9.5
通讯作者：
Hong, Jinkee
中科院分区：
文献类型：
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作者：
Choi, Daheui;Park, Jaeseong;Hong, Jinkee
据报道，细胞包封具有细胞保护作用，能更好地维持细胞存活。与其他研究相比，我们的报告表明，在间充质干细胞（MSCs）上沉积的两种主要生物大分子，ⅰ型胶原（Col）和透明质酸（HA）并没有完全阻断细胞膜表面。相反，TEM观察和基于定量表面标记的FACS分析证实，相当数量的表面仍然未覆盖或仅轻微覆盖。尽管这种结构具有开放性和灵活性，但多层Col/HA膜在无附着培养条件下显著提高了细胞存活率。干细胞特性方面，MSCs在膜沉积后仍表现出功能性细胞活性，其集落形成活性和体外成骨分化证明了这一点。Col/HA多层膜可以提供细胞保护作用并诱导成骨分化，而不会恶化或抑制细胞附着，表明该技术可以成为调节干细胞活性的有价值的工具。
Cell encapsulation has been reported to convey cytoprotective effects and to better maintain cell survival. In contrast to other studies, our report shows that the deposition of two major biomacromolecules, collagen type I (Col) and hyaluronic acid (HA), on mesenchymal stem cells (MSCs) does not entirely block the cell plasma membrane surface. Instead, a considerable amount of the surface remained uncovered or only slightly covered, as confirmed by TEM observation and by FACS analysis based on quantitative surface labeling. Despite this structure showing openness and flexibility, the multilayer Col/HA films significantly increased cell survival in the attachment deprived culture condition. In terms of stem cell characteristics, the MSCs still showed functional cell activity after film deposition, as evidenced by their colony-forming activity and in vitro osteogenic differentiation. The Col/HA multilayer films could provide a cytoprotective effect and induce osteogenic differentiation without deteriorating effect or inhibition of cellular attachment, showing that this technique can be a valuable tool for modulating stem cell activities.