Snake venom phosphodiesterase: simple purification with Blue Sepharose and its application to poly(ADP-ribose) study.
Snake venom phosphodiesterase: simple purification with Blue Sepharose and its application to poly(ADP-ribose) study.
复制标题
蛇毒磷酸二酯酶：用蓝色琼脂糖进行简单纯化及其在聚（ADP-核糖）研究中的应用。
DOI：
10.1016/0006-291x(78)91321-9
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发表时间：
1978
影响因子：
3.1
通讯作者：
O. Hayaishi
中科院分区：
文献类型：
--
作者：
J. Oka;K. Ueda;O. Hayaishi
本文报道了一种快速纯化蛇毒磷酸二酯酶的方法。从商业制剂的酶的60倍纯化是在一个单一的步骤中实现的，产率为60%。纯化的酶制剂基本上不含磷酸酶活性，并在SDS-聚丙烯酰胺凝胶电泳上显示出主要的蛋白质条带。聚（ADP-核糖）的链长分析证实了这种技术的实用性。
A rapid method of purifying snake venom phosphodiesterase has been developed using Blue Sepharose or blue dextran/Sepharose as an affinity adsorbent. A sixty-fold purification of the enzyme from commercial preparations is achieved in a single step with a yield of 60%. The purified enzyme preparation is essentially free from phosphatase activities and exhibits a major protein band on SDS-polyacrylamide gel electrophoresis. Chain length analysis of poly(ADP-ribose) exemplifies the usefulness of this technique.