The neuropeptide SIFamide in the brain of three cockroach species
The neuropeptide SIFamide in the brain of three cockroach species
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DOI：
10.1002/cne.23910
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发表时间：
2016-05-01
影响因子：
2.5
通讯作者：
Stengl, Monika
中科院分区：
文献类型：
--
作者：
Arendt, Andreas;Neupert, Susanne;Stengl, Monika
SIFamide在不同昆虫脑中的序列和分布模式是高度保守的。作为一般规则，至少有四个突出的SIFamide免疫反应性胞体发生在大脑间部。它们遍布大脑和腹神经索。尽管SIFamide与果蝇的交配和睡眠调节有关，但这种肽的其他功能在很大程度上仍然未知。为了确定SIFamide是否在蟑螂的昼夜节律系统中发挥作用，我们研究了SIFamide在Rhyparobia（= Leucophaea）maderae（Blaberidae），Periplaneta americana（Blattidae）和Therea petiveriana（Polyphagidae）中的作用。基质辅助激光解吸/电离-飞行时间（MALDI-TOF）质谱分析显示，在三个物种相同的SIFamide序列（TYRKPPFNGSIFamide）。除了大脑间部（第1组）中的4个大免疫反应细胞外，在大脑间部（第2组）、上级中脑（第3组）和外侧前脑（第4组）中还检测到较小的S异丙酰胺免疫反应胞体。仅美洲大蠊视叶（第5组）和后前脑（第6组）中的其他细胞被染色。几乎整个前脑充满了一个串珠状的网络SIFamide免疫反应过程，特别是强烈侵入中央体的上部单位。双标记实验没有证实与-氨基丁酸（GABA）或昼夜节律偶联肽色素分散因子（PDF）的共定位。与蝗虫相反，SIFamide和组胺免疫反应性的共定位发生在第1组，但在第4组细胞。由于副髓质显示SIFamide免疫反应性和注射SIFamide延迟运动活动的昼夜节律的时间依赖性，SIFamide起着作用的昼夜节律系统的蟑螂。(c)2015 Wiley Periodicals，Inc.
The sequence as well as the distribution pattern of SIFamide in the brain of different insects is highly conserved. As a general rule, at least four prominent SIFamide-immunoreactive somata occur in the pars intercerebralis. They arborize throughout the brain and the ventral nerve cord. Whereas SIFamide is implicated in mating and sleep regulation in Drosophila, other functions of this peptide remain largely unknown. To determine whether SIFamide plays a role in the circadian system of cockroaches, we studied SIFamide in Rhyparobia (= Leucophaea) maderae (Blaberidae), Periplaneta americana (Blattidae), and Therea petiveriana (Polyphagidae). Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry revealed identical SIFamide sequences (TYRKPPFNGSIFamide) in the three species. In addition to four large immunoreactive cells in the pars intercerebralis (group 1), smaller SIFamide-immunoreactive somata were detected in the pars intercerebralis (group 2), in the superior median protocerebrum (group 3), and in the lateral protocerebrum (group 4). Additional cells in the optic lobe (group 5) and posterior protocerebrum (group 6) were stained only in P. americana. Almost the entire protocerebrum was filled with a beaded network of SIFamide-immunoreactive processes that especially strongly invaded the upper unit of the central body. Double-label experiments did not confirm colocalizations with -aminobutyric acid (GABA) or the circadian coupling peptide pigment-dispersing factor (PDF). In contrast to locusts, colocalization of SIFamide and histamine immunoreactivity occurred not in group 1, but in group 4 cells. Because the accessory medulla displayed SIFamide immunoreactivity and injections of SIFamide delayed locomotor activity rhythms circadian time-dependently, SIFamide plays a role in the circadian system of cockroaches. (c) 2015 Wiley Periodicals, Inc.