Sequencing of the chloroplast genomes of cytoplasmic male-sterile and male-fertile lines of soybean and identification of polymorphic markers
Sequencing of the chloroplast genomes of cytoplasmic male-sterile and male-fertile lines of soybean and identification of polymorphic markers
复制标题
大豆细胞质雄性不育系和雄性可育系叶绿体基因组测序及多态性标记鉴定
DOI：
10.1016/j.plantsci.2014.09.005
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发表时间：
2014-12-01
期刊：
影响因子：
5.2
通讯作者：
Dong, Yingshan
中科院分区：
文献类型：
--
作者：
Lin, Chunjing;Zhang, Chunbao;Dong, Yingshan
RN型细胞质雄性不育(CMS)系统用于培育第一个商品化杂交大豆Hybsoy-1，随后已被用于产生几乎所有已发布的大豆杂交种。用常规杂交方法划分不育(S)和正常可育(F)细胞质需要3年以上的时间，但利用细胞器DNA基分子标记可以快速地进行这种划分。杂交大豆不育系和保持系除育性外，其他农艺性状基本相同。因此，很难通过常规肉眼观察在田间种植和育种期间收获过程中出现的混合物中区分它们。在本研究中，我们对F-和S细胞质大豆的叶绿体DNA进行了下一代测序，组装和注释了基因组，并鉴定了区分它们的多态性。F和S细胞质的叶绿体DNA在大小(152,215和152,222碱基对)和GC含量(35.37%)方面非常相似。在基因编码区的23个共享SNP中，我们发现了4个可与限制性内切酶结合用于区分S和F细胞质的SNP。虽然细胞质雄性不育可能与线粒体DNA有关，但线粒体和叶绿体DNA的母体传递允许利用这两个基因组中的任何一个基因组的多态来划分大豆细胞质，从而帮助杂交大豆品种的发展。(C)2014爱思唯尔爱尔兰有限公司。保留所有权利。
The RN-type cytoplasmic male sterility (CMS) system used to develop Hybsoy-1, the first commercial hybrid soybean, has been subsequently applied to generate nearly all released soybean hybrids. Although more than 3 years are needed to classify sterile (S) and normal male-fertile (F) cytoplasms by conventional crossing, such classifications can be performed rapidly using organellar DNA-based molecular markers. Except for fertility, the agronomic traits of CMS hybrid soybean sterile and maintainer lines are identical. Consequently, it is difficult to distinguish them by routine visual inspection in the mixture arising in the course of field planting and harvesting during breeding. In this study, we performed next-generation sequencing of chloroplast DNAs of F- and S-cytoplasmic soybeans, assembled and annotated the genomes, and identified polymorphisms distinguishing them. Chloroplast DNAs of F and S cytoplasms were very similar in size (152,215 and 152,222 base pairs) and GC contents (35.37%). Among 23 shared SNPs in gene coding regions, we identified four that could be used in conjunction with restriction endonucleases to distinguish S and F cytoplasms. Although CMS is likely associated with mitochondrial DNA, maternal transmission of mitochondrial and chloroplast DNAs allows polymorphisms in either genome to be used to classify soybean cytoplasms, aiding hybrid soybean cultivar development. (C) 2014 Elsevier Ireland Ltd. All rights reserved.