In vivo RNA interference of BmNHR96 enhances the resistance of transgenic silkworm to BmNPV
In vivo RNA interference of BmNHR96 enhances the resistance of transgenic silkworm to BmNPV
复制标题
BmNHR96的体内RNA干扰增强转基因蚕对BmNPV的抵抗力
DOI：
10.1016/j.bbrc.2017.09.022
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发表时间：
2017
影响因子：
3.1
通讯作者：
Pan MH
中科院分区：
文献类型：
--
作者：
Yang Ji-gui;Liu Tai-hang;Dong Xiao-long;Wu Yun-fei;Zhang Qian;Zhou Liang;Chen Peng;Lu Cheng;Pan Min-hui;Lu Cheng;Pan Min-hui;Liu Tai-hang;Lu C;Pan MH
我们以前发现了一个核激素受体基因BmNHR 96，它能促进家蚕核型多角体病毒（BmNPV）进入家蚕细胞。为了创造一种抗病毒的家蚕品系以更好地生产蚕丝，我们使用RNAi来下调家蚕幼虫中的BmNHR 96。我们对获得的BmNHR 96-RNAi蚕株（NHR）进行了筛选，并初步探讨了其体内抗病毒机制。结果表明，家蚕幼虫经口服ODV和BV感染后，NHR株的存活率高于大造株。更重要的是，转基因系的经济特征（产丝量）保持不变。这些结果表明，BmNHR 96的RNAi可能是提高家蚕对BmNPV感染耐受性的有效途径。
We previously identified a nuclear hormone receptor gene,BmNHR96, which promotesBombyx morinucleopolyhedrovirus (BmNPV) entry into silkworm cells. In an attempt to create an antiviral silkworm strain for better silk production, we used RNAi to downregulateBmNHR96in silkworm larvae. We screened the resultingBmNHR96-RNAi silkworm strain (NHR) and also explored the antiviral mechanismin vivo. We found that the survival rate of theNHRstrain was higher than that of theDazaostrain, when silkworm larvae were infected with BmNPV via oral ODV infection and BV injection. More importantly, the economic characteristics (silk yield) of the transgenic line remained unchanged. These findings reveal that RNAi ofBmNHR96could be an effective way to enhance the tolerance ofB. morito BmNPV infection.