Ameliorative effect of kefir against γ-irradiation induced liver injury in male rats: impact on oxidative stress and inflammation
Ameliorative effect of kefir against γ-irradiation induced liver injury in male rats: impact on oxidative stress and inflammation
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DOI：
10.1007/s11356-020-09833-7
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发表时间：
2020-06-25
影响因子：
5.8
通讯作者：
Abd El-Rahman, Omnia
中科院分区：
文献类型：
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作者：
Ali, Ola Sayed M.;Amin, Nour El-Din;Abd El-Rahman, Omnia
电离辐射是一把双刃剑，因为它对人类健康既有好处，也有风险。因此，保护人体器官免受辐射的有害影响是研究人员的一个重要关切。开菲尔作为益生菌的良好来源，由于其抗氧化、抗炎和免疫调节的特性，在保护性医学中受到越来越多的关注。因此，本研究计划研究开菲尔对γ辐射诱导的肝毒性的保护作用。将32只雄性大鼠分为4组：（I）对照组，（II）口服开菲尔（5 ml/kg体重）28天，（III）暴露于全身γ射线（6.5戈伊）以诱导肝毒性，（IV）用开菲尔预处理21天，然后暴露于γ射线，随后进行7天的开菲尔治疗。在实验结束时，评估全血细胞计数（CBC）、肝功能和血脂谱。此外，脂质过氧化，一氧化氮含量和内源性抗氧化剂的水平，除了铜，铁和钙的浓度在肝组织中进行了测量。同时检测单核细胞趋化蛋白-1（MCP-1）和核因子-κ（NF-κ B）相关基因表达。结果表明，口服开菲尔可显著减轻辐射引起的肝脏组织学改变、肝功能损害和血脂异常。此外，开菲尔显着改善氧化应激状态，并出现抑制诱导的炎症。本研究提供了开菲尔对γ射线肝毒性的可能的对抗作用。这可以集中在开菲尔应用作为预防性治疗的益处，以限制放射治疗期间的肝脏炎症。
Ionizing radiation is a double-edged sword because of its benefits and risks to human health. Therefore, protecting human organs from harmful effects of radiation is an important concern of researchers. Kefir, as a good source of probiotics, received growing interest in protective medicine owing to its antioxidant, anti-inflammatory, and immunomodulatory properties. Thus, this study was planned to investigate the protective role of kefir against gamma-radiation-induced hepatotoxicity. Thirty-two male rats were distributed in four groups: (I) control, (II) received Kefir orally (5 ml/kg body weight) for 28 days, (III) exposed to whole body gamma-irradiation (6.5 Gy) to induce hepatotoxicity, and (IV) was pretreated with kefir for 21 days then exposed to gamma-irradiation followed by 7 days of kefir treatment. At the end of the experiment, complete blood picture (CBC), liver function, and lipid profile were estimated. Furthermore, levels of lipid peroxidation, nitric oxide content, and endogenous antioxidants, in addition to concentrations of copper, iron, and calcium were measured in liver tissue. Furthermore, monocyte chemoattractant protein-1 (MCP-1) and relative gene expression of nuclear factor kappa (NF-kappa B) were assessed. The results revealed that oral administration of kefir significantly reduced the radiation-induced hepatic histological alterations, hepatic function impairment, and dyslipidemia. Moreover, kefir notably ameliorated the state of oxidative stress and appeared to inhibit the induced inflammation. This study provides a possible counteracting role of kefir against hepatotoxicity induced gamma-radiation. This can focus the benefit of kefir application as a prophylactic treatment to limit hepatic inflammation during radiotherapy.